Assessing the Relationship between Color Values (L*, a* and b*) and Paraoxon-Ethyl Pesticide Residue in Agricultural Products with Acetylcholinesterase
Article Sidebar
Main Article Content
Abstract
Organophosphate are widespread useful in agriculture due to highly effective for the protection / eradication of insects and pests. Thailand is stepping into an aging society in 2021, therefore the healthier food such as fruit and vegetable are influence of living life. However, there are a lot of problems from the use of chemicals in the cultivation process, that are chemical residues in products affected too harmful to consumers. The objective of our research study to find optimize condition and efficiency of Acetylcholinesterase (ACHE) and Ellman’s reagent to check the quantity of Paraoxon Ethyl in the organophosphate group residues in crops. With the mechanism of color change from yellow to transparent color, that is affected due to the quantity of Paraoxon Ethyl at the concentration of 0.2, 0.6 and 1 ppm respectively. The CIE L * a * b * color system is used to process color changes due to the small changes our eyes can't see, that found the correlations between b * values shown negative values. The result shown the values of color tends to approach the value of blue due to the increased concentration of Paraoxon Ethyl in the reference solution. Therefore, b * value can be used to analyze Paraoxon Ethyl at the concentration of 0.2, 0.6 and 1 ppm in the samples, that is expressed as a linear equation with a value of 0.97, 0.9453, and 0.972 respectively. This study is a preliminary evaluation to add a useful alternative to the color analyzer L * a * and b * to represent the analysis of pesticides in vegetables/fruits high accuracy and fast.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Articles in this journal are copyrighted by the x may be read and used for academic purposes, such as teaching, research, or citation, with proper credit given to the author and the journal.use or modification of the articles is prohibited without permission.
statements expressed in the articles are solely the opinions of the authors.
authors are fully responsible for the content and accuracy of their articles.
other reuse or republication requires permission from the journal."
References
ปราณี พัฒนพิพิธไพศาล. (2556). เอนไซม์เทคโนโลยี. กรุงเทพฯ : จุฬาลงกรณ์มหาวิทยาลัย.
Arduini, F., et al. (2005). Extraction and detection of pesticides by cholinesterase inhibition in a two-phase system : A strategy. Analytical Letters, 38(11), 1703-1719.
Beugnet, F. & Franc M. (2012). Insecticide and acaricide molecules and/or combinations to prevent pet infestation by ectoparasites. Trend in Parasitology, 28, 267-279.
Bora, G.C., et al. (2018). Image processing analysis to track color changes on apple and correlate to moisture content in drying stages. Food Quality and Safety, 2, 105-110.
Cayless, M.A., & Marsden, A.M. (1993). A manual of lamp and lighting. (3rd ed.). London : Edward Arnold.
Eleršek, T. & Filipi č, M. (2011). Organophosphorus pesticides mechanisms of their toxicity. In Pesticides-The Impacts of Pesticides Exposure. 243-260. IntechOpen. DOI : 10.5772/14020.
Ellman, G.L., et al. (1961). A new and rapid colorimetric determination of Acetylcholinesterase activity. Biochemical Pharmacology, 7, 88-95.
Hansen, R., Østergaard, H., Nørgaard, P., & Winther, J. (2007). Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4 '-dithiodipyridine. Analytical Biochemistry, 363(1), 77-82.
Jensen, J.R. (2005). Introductory digital image processing: A remote sensing perspective. (3rd ed.) Upper Saddle River : Prentice Hall.
Kubiley, G. et al. (2013). Selective optical sensing of biothiols with Ellman's reagent: 5,5'-Dithio-bis (2-nitrobenzoic acid)-modified gold nanoparticles. Analytica Chimica Acta, 794, 90-98.
Minolta. (1997). Minolta Operator’ s Manual for Photocopier EP5325. Technical Manual Minolta Camera. Business Equipment Division. Osaka, Japan : Minolta.
Nabeshima, T., et al. (2003). An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase in a Japanese encephalitis vector mosquito, Culex tritaeniorhynchus. Biochemical and Biophysical Research Communications, 313, 794-801.
Pohanka, M., et al. (2011). Assessment of acetylcholinesterase activity using indoxylacetate and comparison with the standard Ellman’s method. Molecular Sciences, 12, 2631-2640.
R.W.G. Hunt. (1993). Measuring Colour. (2nd ed.). London : The City University.
Smulders, C.J.G.M., et al. (2003). Selective effects of carbamate pesticides on rat neuronal nicotinic acetylcholine receptors and rat brain acetylcholinesterase. Toxicology and Applied Pharmacology, 193, 139-146.
Suwan, T. & Wongwat, S. (2011). Development of jackfruit seed cracker, Research report from Faculty of Agro-Industry. Bangkok : King Mongkut's University of Technology North Bangkok.
Walker, C.H., et al. (2006). Principle of Ecotoxicology. USA : Taylor & Francis.
Zhang, S.P., et al. (2008). Study of enzyme biosensor based on carbon nanotubes modified electrode for detection of pesticides residue. Chinese Chemical Letters, 19, 592-594.
