Sensor for Homocysteine Measurement in the Indicate Cardiovascular Disease
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Abstract
Cardiovascular diseases are top-mortal chronic disease. The indicator of cardiac diseases that can show myocarditis is homocysteine (Hcy) because of its specificity and susceptibility. Currently, the method for quantity analysis of homocysteine uses a high-performance liquid chromatography system that uses fluorescence as a detector or an analysis by immune system using antibodies to homocysteine. These methods acceptable exact homocysteine analysis results but the disadvantage is that it takes time to analyze, the tool is expensive, and cannot be used in the field or at medical service points. Thus, in this research, an artificial bioreceptor has been synthesized for binding homocysteine by the surface modification of the electrode with composite material of multi-wall carbon nanotubes at the amount of 0.25, 0.5, 0.75 and 1 gram together with chitosan at the amount of 0.2 gram in every ratio. The SEM has been confirm the surface modification with nanocomposite and the analysis of electrochemical properties with Cyclic voltammetry (CV). Results revealed that the surface modification with nanocomposite is capable of effective homocysteine detection, stability and fast analysis time. This sensor design will be employed as a new bioreceptor for detect complex protein structures on serum samples.
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