Selection of Native Entomopathogenic Nematode from Southern Thailand for Developing EPN-based Bioproduct
Keywords:
Entomopathogenic nematode, EPN-based bioproduct , biological controlAbstract
Entomopathogenic nematode (EPN) is one of the important biological control agents for controlling key agricultural insect pests, ensuring safety for non-target organisms and the environment. This study was conducted to screen the effective local EPNs from soil samples collected from four provinces in southern Thailand. The results found that EPN from Pattani province ultimately killed 100% of the tested larvae, wax moth (Galleria mellonella), within 2 days. From the visible symptoms of infected wax moth and the morphological characteristics of selected EPN under a microscope and morphometric study, it could be identified that the selected EPN was Heterorhabditis sp. Subsequently, Heterorhabditis sp. was developed into an EPN-based bioproduct. Regarding the Plackett−Burman design, evaluating the solubility of the product, viability of EPN, and host infection ability resulted in selecting high-affected formulation components, including kaolin, Tween 20, CMC, formalin, and sodium polyacrylate.
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