Oil Palm Progeny Test: SNP Markers in the Parents of Hybrid Crosses of the Third Oil Palm Breeding Project of the Department of Agriculture

Authors

  • Suvimon Konlasuk Suratthani Oil Palm Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture
  • Ornrat Wongsri Suratthani Oil Palm Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture
  • Sujittra Promchaue Suratthani Oil Palm Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture
  • Tuenjit Pethrrun Suratthani Oil Palm Research Center, Field and Renewable Energy Crops Research Institute, Department of Agriculture

Keywords:

SNP markers, Dura, Tenera , MADS-box gene, oil palm breeding

Abstract

The third oil palm breeding project conducted by the Department of Agriculture aims to improve fresh fruit bunch (FFB) and oil yield. In this study, 23 lines of maternal Dura and 17 lines of paternal Tenera were selected to generate hybrid crosses (Dura × Tenera, D × T). The objective was to detect single nucleotide polymorphisms (SNPs) at specific positions within the MADS-box gene in parental palms and to utilize these SNP markers for identifying Pisifera pollen donors and validating the genetic identity of Tenera hybrid seeds. SNP detection at positions SNPENGC (272), SNPTaYa (279), and SNPLaAV (308) was performed using the TaqMan real-time PCR technique. The maternal Dura palms showed no nucleotide variation at these positions, consistently displaying T, A, and C at SNPENGC, SNPTaYa, and SNPLaAV, respectively. In contrast, the paternal Tenera lines exhibited SNP polymorphisms: eight lines (398/925T 1446/412T 154/1233T 2/496T 4/1075T 5/170T 9/908T and 10/815T) had an A/T genotype at SNPTaYa, five lines (102/417T 197/654T 71/563T 1/481T and 3/359T) had a T/C genotype at SNPENGC, and four lines (49/86T 520/184T 6/207T and 8/1027T) had a C/A genotype at SNPLaAV. These SNP markers are promising tools for improving the precision of Pisifera parent selection and hybrid seed validation in oil palm breeding programs.

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Published

2025-12-22

How to Cite

Konlasuk, S., Wongsri, O. ., Promchaue, S. ., & Pethrrun, T. . (2025). Oil Palm Progeny Test: SNP Markers in the Parents of Hybrid Crosses of the Third Oil Palm Breeding Project of the Department of Agriculture. AgriScience and Society Journal, 1(2), 26–36. retrieved from https://li02.tci-thaijo.org/index.php/NRJ/article/view/1169

Issue

Vol. 1 No. 2 (2025): July - December

Section

Research Articles

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