Isolation of Thermostable Amylase-Producing t Bacteria from Soil
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Abstract
The aim of this research was to isolate thermostable amylase-producing bacteria from soil. Soil samples were collected from Pranakpok, Faculty of Liberal Arts and Science building and entrance to Roi Et Rajabhat University. One gram of each soil samples was added into Nutrient broth (NB) medium containing 1% starch and incubated at 37°C for 24 hours before continued culturing at 60°C for 48 hours. The amylase activity was screened on nutrient agar containing 1% starch using iodine solution. Three isolates were survived and presented the clearing zone of amylase activities were selected and named AN01 AN02 and AN03. Amylase activities of 3 isolated were determined at 50 60 70 80 90 and 100°C using starch solution (pH 7.0) as substrate. The result found that AN02 exhibited the highest amylase activities at 60°C (0.183 U/ml). Result of bacterial identification using partial 16S rRNA indicated that AN02 was Bacillus aryabhattai at 99.5% identity using EzBioCloud data base.
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