Determination of Carbamates Pesticide Residue in Vegetables by Acetylcholinesterase for Application Sensors

Abstract

Carbamates group is one of the most widely used insecticides, that often-accumulated residue in fruits and vegetables. The residue of that chemical on fruits and vegetables directly affects the health of consumers such as the nervous system, muscle system, and affect the occurrence of various diseases. The research team has the objective of studying the measurement of Carbaryl substance that is in the Carbamates group, it residue in fruits and vegetables. To determination of the amount of Carbaryl by based on the reaction principle of Acetylcholinesters Enzyme (AChE) to turns from a clear solution to a yellow solution when Carbaryl is detected, it will inhibit color changes that occur. The process of detecting color changes uses Spectrophotometer Colorimeter by CIE L* a* b* system for analysis and measurement of that result. Therefore, the amount of Carbaryl which has the best effect on discoloration were 0.2, 0.6 and 1.0 ppm, respectively. That reaction time is only 3 minutes,which takes less time than testing with other methods due to carbaryl can quickly inhibit the reaction of the Acetylcholinesters Enzyme (AChE). From the above test results, we hope to develop into a portable device for rapid analysis of the amount of Carbaryl less than 1 ppm.