Effects of Lactic Acid Bacteria Supplementation on Nutrient Intake, Milk Yield and Milk Chemical Composition of Dairy Cows
Keywords:
Lactic acid bacteria, Milk yeild, Milk composition, Nutrient intake, Dairy cowAbstract
This study aims to evaluate the effects was supplemented with lactic acid bacteria (LAB) in dairy cow feed at a level of 200 grams per day on nutrient intake, milk yield, and milk chemical composition. The experiment used 8 crossbred Holstein Friesian dairy cows, divided into a control group (not supplemented with LAB) and an experimental group (supplemented with LAB 200 grams/day). The experiment was conducted for 48 days, including a 13-day adaptation period and a 35-day data collection period, which was divided into five 7-day collection periods. The LAB supplemented used in this study consisted of Streptococcus thermophilus, Lactobacillus acidophilus, and Bifidobacterium, with a live microbial concentration of approximately 1.0 × 109 CFU/g. The results showed that LAB supplementation did not have a statistically significant effect (P>0.05) on body weight, nutrient intake, milk yield, or milk chemical composition. However, future studies should investigate the effect of LAB supplementation at different levels and in early-lactation cows with high energy demands to clearly assess its potential for improving dairy cow production performance in tropical conditions.
References
Anantasook, N., Thongpea, S., Cherdthong, A., Gunun, P., Gunun, N., Wanapat, M., & Kang, S. (2021). Effect of tannin sources on
in vitro ruminal fermentation, methane production, and ruminal microbial population. Animals, 11, 2308. https://doi.org/10.3390/ani11082308
AOAC. (2005). Official methods of analysis of AOAC International (18th ed.). Association of Official Analytical Chemists. Bach, A.,
López-García, A., González-Recio, O., Milán, M. J., Babot, F., & Devant, M. (2022). Changes in the rumen microbiome and metabolome of beef cattle in response to diet. Frontiers in Veterinary Science, 9, 829807. https://doi.org/10.3389/fvets.2022.829807
Chaiyasut, C., & Sirilun, S. (2022). Selection criteria and characterization of potential probiotic strains for ruminant applications.
Journal of Applied Microbiology, 132, 2845–2857. https://doi.org/10.1111/jam.15393
Doyle, N., Mbandlwa, P., Kelly, W. J., Attwood, G., Li, Y., Ross, R. P., Stanton, C., & Leahy, S. (2019). Use of lactic acid bacteria to
reduce methane production in ruminants: A critical review. Frontiers in Microbiology, 10, 2207.
https://doi.org/10.3389/fmicb.2019.02207
Fomenky, B. E., Do, D. N., Talbot, G., Chiquette, J., Bissonnette, N., Chouinard, Y. P., Lessard, M., & Ibeagha-Awemu, E. M.
(2018). Direct-fed microbial supplementation influences the bacteria community composition of the gastrointestinal tract
of pre- and post-weaned calves. Scientific Reports, 10, 14147. https://doi.org/10.1038/s41598-020-70906-1
Golder, H. M., & Lean, I. J. (2023). Future directions for improving dairy cattle health and performance using direct-fed microbials.
Journal of Dairy Science, 106, 2384–2407. https://doi.org/10.3168/jds.2022-22608
Golder, H. M., Hodge, A., & Lean, I. J. (2020). Effects of in utero and early-life conditions on adult health and disease: The role of
nutrition. Animal, 14, 435–445. https://doi.org/10.1016/j.animal.2019.10.040
Heinrichs, A. J., Erb, H. N., Rogers, G. W., Cooper, J. B., & Jones, C. M. (2007). Variability in Holstein heifer heart girth
measurements and comparison of prediction equations for live weight. Preventive Veterinary Medicine, 78, 333–338.
https://doi.org/10.1016/j.prevetmed.2006.10.004
Jian, H., Ding, P., Li, X., Zhang, H., Xie, H., Wang, L., Shen, X., & Geng, F. (2021). Regulation of rumen fermentation pattern,
microbiota, and metabolites of Holstein heifers supplemented with Bacillus subtilis and Lactobacillus casei. Applied
Microbiology and Biotechnology, 105, 7783–7795. https://doi.org/10.1007/s00253-021-11611-3
Liu, Y., Hou, T., Song, X., Wang, T., Dong, X., Yao, J., & Liu, F. (2022). Effects of combined Bacillus and lactic acid bacteria in
rumen fermentation, milk production, ruminal microbiome, and metabolome in Holstein dairy cows. Journal of Animal
Science and Biotechnology, 13, 112. https://doi.org/10.1186/s40104-022-00741-5
Ma, Z. Z., Cheng, Y. Y., Wang, S. Q., Ge, J. Z., Shi, H. P., & Kou, J. C. (2020). Positive effects of dietary supplementation of three
probiotics on milk yield, milk composition and intestinal flora in Sannan dairy goats varied in kind of probiotics. Journal of
Animal Physiology and Animal Nutrition, 104, 44–55. https://doi.org/10.1111/jpn.13217
Nocek, J. E., & Kautz, W. P. (2006). Direct-fed microbial supplementation on ruminal digestion, health, and performance of pre- and
postpartum dairy cattle. Journal of Dairy Science, 89, 260–266. https://doi.org/10.3168/jds.S0022-0302(06)72085-8
Ottenstein, T. M., Myers, W. A., Martin, J. A., & Bremer, V. R. (2022). Direct-fed microbial supplementation on health,
performance, fermentative profiles, immunological parameters, and economics of dairy cows: A meta-analysis. Journal of
Dairy Science, 105, 7539–7557. https://doi.org/10.3168/jds.2021-21565
Philippeau, C., Lettat, A., Martin, C., Silberberg, M., Morgavi, D. P., Ferlay, A., Berger, C., & Nozière, P. (2021). Effects of bacterial
probiotic on ruminal fermentation and microbial populations, and milk yield and composition in dairy cows. Livestock
Science, 245, 104299. https://doi.org/10.1016/j.livsci.2021.104299
Philippeau, C., Lettat, A., Martin, C., Silberberg, M., Morgavi, D. P., Ferlay, A., Berger, C., & Nozière, P. (2017). Effects of bacterial
probiotic on ruminal fermentation and microbial populations, and milk yield and composition in dairy cows. Livestock
Science, 199, 79–87. https://doi.org/10.1016/j.livsci.2017.03.011
Steel, R. G. D., & Torrie, J. H. (1980). Principles and procedures of statistics: A biometrical approach (2nd ed.). McGraw-Hill.
Uyeno, Y., Shigemori, S., & Shimosato, T. (2015). Effect of probiotics/prebiotics on cattle health and productivity.
Microbes and Environments, 30, 126–132. https://doi.org/10.1264/jsme2.ME14176
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Symposium: Carbohydrate methodology, metabolism, and nutritional
implication in dairy cattle: Methods for dietary fiber and nonstarch polysaccharides in relation to animal. Journal of Dairy
Science, 74, 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Wanapat, M., Cherdthong, A., Phesatcha, K., & Kang, S. (2019). Dietary sources and their effects on animal production and
environmental sustainability. Animal Nutrition, 5, 11–18. https://doi.org/10.1016/j.aninu.2018.11.004
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Faculty of Natural Resources
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Natural Resources and Agricultural Sciences Journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence, unless otherwise stated. Please read our Policies page for more information on Open Access, copyright and permissions.
