Genetic Diversity Analysis and Identification of Plai-Dum (Zingiber ottensii Valeton) in Thailand with Molecular Markers

Authors

  • Vipavee Chanroj Biotechnology Research and Development Office, Department of Agriculture, Rangsit, Thanyaburi, Pathum Thani 12110
  • Aphinya Wongpia Biotechnology Research and Development Office, Department of Agriculture, Rangsit, Thanyaburi, Pathum Thani 12110
  • Pornpayung Kongsuwan Yala Horticulture Research Center, Department of Agriculture, Than To, Than To, Yala 95150

Keywords:

Plai-Dum, SSR, genetic diversity, identification

Abstract

Plai-Dum (Zingiber ottensii Valeton) is a local medicinal herb primarily found in southern Thailand belonging to the Zingiberaceae family. The plant is characterized by zerumbone as a major component in its essential oils, which exhibits antimicrobial, anticancer, and anti-inflammatory properties. This study investigated the genetic diversity and classification of Plai-Dum using molecular markers. A total of 34 accessions of Z. ottensii from Thailand and 19 accessions of other Zingiber species were collected and analyzed.  Fifty-eight simple sequence repeat (SSR) primer pairs were screened, and 33 pairs successfully amplified DNA through PCR.  Among these, 14 pairs of SSR primers showed polymorphism in Z. ottensii and related species The analysis detected a total of 150 alleles with an average of 10.71 alleles per primer. Cluster analysis based on genetic relationships using UPGMA classified all accessions into three distinct clusters. Cluster 1 consisted exclusively of Z. ottensii accessions. Cluster 2 included Z. zerumbet, Z. flavomaculosum, Z. montanum, and Z. parishii, while cluster 3 comprised Z. officinale accessions. The genetic similarity coefficient ranged from 0.57 to 1.00, with an average of 0.83. Notably, all Z. ottensii accessions showed the highest genetic similarity coefficient (1.00), indicating a common origin and identical clones with the same genetic information within Thailand. This genetic uniformity likely results from the common practice of asexual propagation through rhizomes, which produces genetically identical offspring. The study identified ZOSSR25 as an effective marker for distinguishing Z. ottensii from other Zingiber species, demonstrating the utility of SSR markers as rapid and reliable tools for identifying seedlings in plantation establishment.

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Published

2025-06-23

How to Cite

Chanroj, V., Wongpia, A. ., & Kongsuwan, P. . (2025). Genetic Diversity Analysis and Identification of Plai-Dum (Zingiber ottensii Valeton) in Thailand with Molecular Markers. Natural Resources and Agricultural Sciences Journal, 1(1), 22–32. retrieved from https://li02.tci-thaijo.org/index.php/NRJ/article/view/1144

Issue

Vol. 1 No. 1 (2025): January - June

Section

Research Articles

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