สารสกัดน้ำเบญจกูลมีผลต่อการแก่ของเซลล์ตับในหนูอ้วนที่มีไขมันสูง: Benjakul Water Extract Affects Liver Cell Aging in Obese Mice with High-fat Diet

Kevalin Vongthoung; Narongsuk Munkong; Achiraya Kamchansuppasin; Atthapan Morchang

Authors

Kevalin Vongthoung Medical Technology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University
Narongsuk Munkong Department of Pathology, School of Medicine, University of Phayao
Achiraya Kamchansuppasin Institute of Nutrition, Mahidol University
Atthapan Morchang School of Medicine, Mae Fah Luang University

Keywords:

benjakul water extract, metabolic syndrome , aging, Senescence-associated beta-galactosidase (SA-β-gal)

Abstract

The Benjakul water extract (BWE) is a traditional remedy in Thailand utilized for managing blood sugar levels. However, there has been limited research into the effects and mechanisms of BWE on the liver. This study aimed to evaluate the anti-aging properties of BWE by assessing the activity of the Senescence-associated beta-galactosidase (SA-β-gal) enzyme in the liver cells of obese mice subjected to a high-fat diet. The study involved 16 male Sprague-Dawley rats, divided into 6 groups of 3 rats each. Following a 4-week period of high-fat diet consumption, the rats' body weight, blood sugar levels, and SA-β-gal immunohistochemistry in the liver were evaluated. Comparing the results with the control group (rats on a normal diet), all rat groups exhibited increased body weight, blood sugar levels, and SA-β-gal staining in the liver, indicating statistical significance. Among the groups fed a high-fat diet, those supplemented with low-dose BWE and high-dose BWE showed reduced SA-β-gal staining. In contrast, the group receiving a high-fat diet along with wild betal leaf bush water extract, and the group receiving a high-fat diet along with Metformin, exhibited increased SA-β-gal staining. Moreover, wild betal leaf bush water extract and Metformin were found to accelerate cellular aging in the liver cells of obese rats receiving a high-fat diet. Conclusion, both low and high doses of BWE demonstrated potential in mitigating liver cell aging in obese rats subjected to a high-fat diet. Furthermore, wild betal leaf bush water extract and Metformin were observed to expedite liver cell aging, suggesting that BWE might possess protective properties against the aging process in liver cells under conditions associated with high levels of dietary fat.

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Benjakul Water Extract Affects Liver Cell Aging in Obese Mice with High-fat Diet