การคัดเลือกเชื้อราเอนโดไฟต์ในเขตป่าอนุรักษ์จังหวัดจันทบุรี  ในการควบคุมเชื้อราสาเหตุโรคในทุเรียน: Screening of Endophytic Fungi from Protected Forest Areas in the Chanthaburi Against Disease in Durians

Titi  Thongkamngam; Sukritta  Anutrakunchai

Authors

Titi Thongkamngam Department of Plant Production and Landscape, Faculty of Agro Industrial Technology, Rajamangala University of Technology Tawan-ok, Chanthaburi
Sukritta Anutrakunchai Department of Plant Production and Landscape, Faculty of Agro Industrial Technology, Rajamangala University of Technology Tawan-ok, Chanthaburi

Keywords:

Endophytic fungi, Forest Conservation, Plant Pathogenic fungi

Abstract

Samples of endophytic fungi were collected from protected forest areas located in Chanthaburi province. The samples were obtained from various plant species including the Zingiber zerumbet, Kaempferia marginata, Barringtonia asiatica, Combretum indicum, Ichnocarpus frutescens, Thunbergia laurifolia and Asplenium nidus. Sixteen types of endophytic fungi are as follows: Penicillium sp., Aspergillus sp., Colletotrichum sp., Pestalotia sp., Fusarium sp. 01-02, Curvularia sp., Bipolaris sp., Fusarium solani, Alternaria sp., Aspergillus niger, Nigrospora sp., Rhizoctonia sp., Sclerotium sp. 01-02, and Trichoderma sp. Subsequently, pathogenic fungi causing fruit rot, leaf spot, and pink disease were identified: Fusarium solani, Lasiodiplodia sp., and Rhizoctonia solani. Furter testing showed that all strains of the endophytic fungi were able to inhibit the growth of these three pathogenic fungi. The endophytic fungus CPB 5 showed inhibition percentages against F. solani, Lasiodiplodia sp., and R. solani equal to 95.4, 96.6, and 98.3 percent, respectively. Molecular analysis confirmed the classification of Trichoderma asperellum up to 100 percent. Conclusion, that the Endophytic fungi in the protected forest areas can be applied to durian production in Chanthaburi.

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Screening of Endophytic Fungi from Protected Forest Areas in the Chanthaburi Against Disease in Durians