The Carotenoids Production from Rhodopseudomonas sp.  OS33-UV13-5 Cultivated in Chitin Medium

Nisa  Krairak; Somchai  Krairak

doi:10.53848/ssstj.v12i2.1013

Authors

Nisa Krairak Burapha University, Chonburi
Somchai Krairak King Mongkut’s Institute of Technology Ladkrabang, Bangkok

DOI:

https://doi.org/10.53848/ssstj.v12i2.1013

Keywords:

Chitin, Carotenoids, Rhodopseudomonas sp, N-acetyl glucosamine

Abstract

In this work, the carotenoids (CD) production of Rhodopseudomonas sp. OS33-UV13-5 in the presence of different chitin forms such as chitin powder, chitin flake, squid pen and squid pen powder was studied for carbon source utilization, respectively. Rhodopseudomonas sp. OS33-UV13-5 showed the ability to degrade chitin. When Rhodopseudomonas sp. OS33-UV13-5 was cultivated in chitin medium containing 10 g/L of squid pen powder as a carbon source, the growth (bacteriochlorophylls content, Bchl) and CD were found at 6.32±0.75 Bchl U_770nm and 23.14±1.86 mg/L, respectively. Then, the optimized culture conditions were examined with various squid pen powder concentrations and light intensities. The results showed that maximum cell growth and CD production were 14.64±1.91 Bchl U_770nm and 45.87±3.85 mg/L, respectively, when cultivated in 30 g/L of squid pen powder under 1500 lux of light intensity at 40^oC for 15 days. The CD production increased by about 2.02-fold. The Rhodopseudomonas sp. OS33-UV13-5 chitinase production displayed a maximum at 9.94±1.08 U/mL on day 9 of cultivation. The accumulation of N-acetyl glucosamine (NAG) was about 1.98±0.32 to 3.38±0.81 mg/L. Future research should emphasize on the fed-batch cultivation to enhance the CD production by maximizing chitin assimilation.

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