Actinomycetes as Effective Biocontrol Agent Against Soft Rot Disease on Choy sum Caused by Erwinia carotovora pv. carotovora
DOI:
https://doi.org/10.53848/ssstj.v12i2.1021Keywords:
Actinomycetes, Erwinia carotovora pv. carotovora, Streptomyces hygroscopicus, Soft rot disease, Biological controlAbstract
Soft rot disease caused by Erwinia carotovora pv. carotovora severely affects Choy sum (Brassica rapa var. parachinensis) production, particularly in tropical regions such as Thailand. Conventional control methods rely heavily on chemical pesticides, raising concerns about environmental sustainability. This study aimed to explore indigenous Actinomycetes from rice field soil in Suphan Buri Province, Thailand, for their potential as biological control agents against soft rot disease and as plant growth promoters. Sixty actinomycete isolates were screened using the agar plug diffusion method, and four isolates (SPB1012, SPB1001, SPB1035, and SPB1022) exhibited significant antagonistic effects against E. carotovora pv. carotovora. Among them, SPB1012 demonstrated the highest inhibitory activity and effectively promoted growth parameters such as shoot height and stem diameter in greenhouse experiments with Choy sum. Molecular characterization revealed 100% sequence similarity between the isolates and Streptomyces hygroscopicus subsp. hygroscopicus NBRC 13472T. These findings suggest that indigenous Actinomycetes from rice fields can serve as promising biocontrol agents for sustainable vegetable production, reducing reliance on chemical pesticides and contributing to environmentally friendly agricultural practices.
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