Study on Intracellular Extracts of Chlorella sp. KLSc61 to Promote Growth of Lactobacillus plantarum JCM 1149

Wipawee Dejtisakdi; Lalita Khanrin; Pawanrat  Photising; Panatda  Phakdeekarngana

doi:10.53848/ssstj.v12i1.1022

Authors

Wipawee Dejtisakdi King Mongkut’s Institute of Technology Ladkrabang
Lalita Khanrin King Mongkut's Institute of Technology Ladkrabang
Pawanrat Photising King Mongkut's Institute of Technology Ladkrabang
Panatda Phakdeekarngana King Mongkut's Institute of Technology Ladkrabang

DOI:

https://doi.org/10.53848/ssstj.v12i1.1022

Keywords:

Chlorella sp. KLSc61, Ethanol, Intracellular extract, Lactobacillus plantarum

Abstract

Chlorella is one of the famous microalga genera used to study in various fields, including functional food. Several studies revealed that its dried biomass could be used as prebiotics to promote the growth of probiotics such as Lactobacillus sp. Using Chlorella dried cell mass might be ineffective while producing on an industrial scale. To find an alternative strategy, we tested if Chlorella crude extract comprised some bioactive compound that could promote the growth of Lactobacillus or not, this led to reduce the amount of Chlorella use. This study aimed to compare fresh and dried Chlorella crude extract to find out which one could be a better use, which was still unclear. We prepared fresh and dried intracellular ethanolic extracts of Chlorella sp. KLSC61, added them into the Lactobacillus plantarum JCM 1149 growth medium (MRS), and measured the growth of L. plantarum JCM 1149. The results showed that all dried crude extracts significantly showed better L. plantarum JCM 1149 growth promotion than fresh ones. The 0.1% (w/v) of dried-ethanolic crude extract could promote growth up to 1.1-1.2-fold compared to controls (MRS and glucose) at 14 and 24 h during the stationary phase. These preliminary results demonstrated that Chlorella sp. KLSC61 crude extracts contained some bioactive compounds that could enhance the growth of L. plantarum JCM 1149, and that compounds might be heat resistant. We propose analyzing the crude extract and finding out a specific compound that could benefit the growth of L. plantarum JCM 1149 for future study.

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