Pulsed electric field (PEF)-assisted Extraction of Phenolic Compounds from Mangosteen Pericarp
Keywords:
Pulsed electric field-assisted extraction, mangosteen pericarp, phenolic content, antioxidant activityAbstract
Mangosteen (Garcinia mangostana Linn.) pericarp is a rich source of phytochemicals. Therefore, It is used as a traditional medicine. This study aims to investigate effects of pulsed electric field (PEF)-assisted extraction of mangosteen pericarp on total phenolic content (TPC) and antioxidant activity using ferric reducing ability power (FRAP) assay. The study conditions were varied by PEF intensity (4, 5 and 6 kV/cm-1) with 1000, 3000 and 5000 pulses. Mangosteen pericarp macerated in water was used as a control. The study revealed that TPC value increased with higher intensity and pulses. The highest TPC was observed, in the pericarp extracted with 6 kV/cm-1 and 5000 pulses, at 0.17 mgGAE/gFW. The PEF-assisted extraction showed 425% increment compared to its untreated counterpart. Similar trend was found with FRAP assay where the pericarp extracted with PEF (6 kV/cm-1, 5000 pulses) showed the highest FRAP value at 0.38 mgGAE/gFW. Moreover, TPC value and FRAP value exhibited positive correlation with r = 0.9869.
References
Alide, T., Wangila, P., & Kiprop, A. (2020). Effect of cooking temperature and time on total phenolic content, total flavonoid content and total in vitro antioxidant activity of garlic. BMC Research Notes, 13, 564.
Boonrat, C., & Indranupakorn, R. (2015). Influence of extraction techniques and solvents on α-mangostin amounts from mangosteen (Garcinia mangostana L.) pericarp, Thai Bulletin of Pharmaceutical Sciences, 10(2), 1-11.
Dobreva, A., Tintchev, F., Dzhurmanski, A., & Toepfl, S. (2013). Effect of pulsed electric fields on distillation of essential oil crops. Comptes rendus de l’Académie bulgare des Sciences: Sciences Mathématiques et Naturelles, 66(9), 1255-1260.
Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M., & Merillon, J. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry, 57(5), 1768-1774.
Folin, O., & Ciocalteu, V. (1927). On tyrosine and tryptophane determinations in proteins. Journal of Biological Chemistry, 73, 627-650.
Mohammad, N. A., Zaidel, D. N. A., Muhamad, I. I., Hamid, M. A., Yaakob, H., & Jusoh, Y. M. M. (2018). Optimization of the antioxidantrich xanthone extract from mangosteen (Garcinia mangostana L. ) pericarp via microwave-assisted extraction. Heliyon, 5, e0257.
Muchtaridi, M., Prasetio, M., Saptarini, N. M., & Saputri, F. A. (2018). High performance liquid chromatography for α- mangostin analysis in mangosteen pericarp extract for routine analysis with photodiode array detector. Rasayan Journal of Chemistry, 11(3), 973-978.
Mulia, K., Hasanah, F., & Krisanti, E. A. (2018). The effect of heating temperature on cytotoxicity and α- mangostin yield: Mangosteen pericarp juice and mangosteen extract. IOP Conference Series: Materials Science and Engineering, 316, 012010.
Satong-aun, W., Assawarachan, R., & Noomhorm, A. (2011). The influence of drying temperature and extraction methods on α- mangostin in mangosteen pericarp. Journal of Food Science and Engineering, 1, 85-92.
Shaimaa, G. A., Mahmoud, M. S., Mohamed, M. R., & Emam, A. A. (2016) . Effect of heat treatment on phenolic and flavonoid compounds and antioxidant activities of some Egyptian sweet and chilli pepper. Natural Products Chemistry & Research, 4(3), 1-6.
