β-glucosidase enzyme screening from various parts of Tabebuia argentea
Keywords:β–glucosidase, Tabebuia argentea, p-nitrophenyl-D-glucopyranoside
The aim of this work was to search for the novel β–glucosidase enzyme from various parts of Tabebuia argentea; flower, flower bud, shoot, seed shank and seed. The enzyme was extracted from the samples by using appropriated buffer. The ammonium sulfate ((NH4)2SO4) salt precipitation in the different salt concentrations was used to initial fractionated purification steps. The monitoring of enzyme activity was carried out by using hydrolysis reaction of glycosidic bond using p-nitrophenyl-D-glucopyranoside (pNPG) as an enzyme substrate. The UV-vis spectroscopy was used to detect the corresponding p-nitrophenylate product under basic condition at 405 nm. The enzyme activity via pNPG hydrolysis of seeds extract was around 15-fold over the other plant part extracts of Tabebuia argentea. This was followed by selected enzyme fraction of the seeds extract subjected to optimum temperature study and temperature for the best enzyme activity was 30-40 °C. The highest enzyme activity fraction will be used for further purification and enzymatic properties test before application as a biocatalyst in biological process.
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