Nutritional Value, Bioactive Compounds, and Antioxidant Activity of Phak-liang (Gnetum gnemon Linn. var. tenerum Markgr.) in Surat Thani Province, Thailand
DOI:
https://doi.org/10.53848/ssstj.v11i2.816Keywords:
Nutritional value, Bioactive compounds, Antioxidant activity, Gnetum gnemon Linn. var. tenerum Markgr.Abstract
The nutritional value, bioactive compounds, and antioxidant activity of Phak-liang (Gnetum gnemon Linn. var. tenerum Markgr.) were investigated. The indigenous vegetables were collected from three districts; Muang, Bannasarn, and Khirirat Nikom in Surat Thani Province. The nutritional value of the samples was carried out according to the methods of AOAC. The moisture contents ranged from 84.72-87.52%, and the ash contents ranged from 0.78-1.07% fresh weight. The percentage of fiber, protein, fat, and carbohydrate in the leafy vegetables were in the range of 3.06-3.81, 3.44-4.68, 0.65-1.08 and 7.49-9.55 fresh weight, respectively. The samples were analyzed by ICP-OES for their mineral determinations. The amounts found in the samples, in descending order were: K > Ca > Mg > Mn > Na> Fe > Zn > Cu. Considering the amounts of minerals found in the vegetable and their recommended daily values by the Bureau of Nutrition (Ministry of Public Health, Thailand), the daily value (DV) percentage of Mn in the samples were relatively high (28.45-96.65%), whereas K, Mg, Ca, Fe, Cu and Zn contributed from 1.29–8.42% of the daily values. The vegetable had negligible amounts of Na (0.03 - 0.07% of the DV). The antioxidant capacities were analyzed using Ferric Reducing Antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results showed that antioxidant activity increased by the concentration of the ethanol extracts. The FRAP values of the extracts ranged from 89.01-160.70 µM FeSO4/100 g dry weight, and the IC50 values calculated for DPPH radical scavenging activities of the extracts ranged from 54.37-60.57 mg/mL. The results suggested that Phak-liang is a potential source of natural phytochemicals and could be used for health promotion in the community.
References
References
Alam, F., Saqib, Q. N., & Ashraf, M. (2017). Gaultheria trichophylla (Royle): a source of minerals and biologically active molecules, its antioxidant and anti-lipoxygenase activities. BMC Complementary and Alternative Medicine, 17, 1-9. doi:10.1186/s12906-016-1511-4
Alfa, H. H., & Arroo, R. R. J. (2019). Over 3 decades of research on dietary flavonoid antioxidants and cancer prevention: What have we achieved? Phytochemistry Reviews, 18, 989-1004. doi:10.1007/s11101-019-09632-0
Benzie, I. F. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. doi:10.1006/abio.1996.0292
Boeing, H., Bechthold, A., Bub, A., Ellinger, S., Haller, D., Kroke, A., … Watzl, B. (2012). Critical review: Vegetables and fruit in the prevention of chronic diseases. European Journal of Nutrition, 51, 637-663. doi:10.1007/s00394-012-0380-y
Bureau of Nutrition. (2020). Dietary reference intake for Thais 2020. Bangkok: Department of Health, Ministry of Public Health.
Charoenteeraboon, J., Ngawhirunpat, T., Chaidedgumjorn, A., Asavapichayont, P., Mahadlek, J., & Tuntarawongsa, S. (2019). Total phenolic content and antioxidant activities of different parts of Hom Thong banana extract and extraction related factors. Thai Bulletin of Pharmaceutical Sciences, 14(2), 47-60.
Isabelle, M., Lee, B. L., Lim, M. T., Koh, W. P., Huang, D., & Ong, C. N. (2010). Antioxidant activity and profiles of common vegetables in Singapore. Food Chemistry, 120, 993-1003.
Jiménez-Aguilar, D. M., & Grusak, M. A. (2017). Minerals, vitamin C, phenolics, flavonoids and antioxidant activity of Amaranthus leafy vegetables. Journal of Food Composition and Analysis, 58, 33-39.
doi:10.1016/j.jfca.2017.01.005
Kato, E., Tokunaga, Y., & Sakan, F. (2009). Stilbenoids isolated from the seeds of melinjo (Gnetum gnemon L.) and their biological activity. Journal of Agricultural and Food Chemistry, 57(6), 2544-2549.
doi:10.1021/jf803077p
Kongkachuichai, R., Charoensiri, R., Yakoh, K., Kringkasemsee, A., & Insung, P. (2015). Nutrients value and antioxidant content of indigenous vegetables from Southern Thailand. Food Chemistry, 173, 838-846. doi:10.1016/j.foodchem.2014.10.123
Latimer, G. W. (2016). Official methods of analysis of AOAC International (20th ed.). Maryland, USA: AOAC International.
Pakdee, N., Poowanna, R., & Prathumtet, J. (2021). Comparison of antioxidant activity and amount of total phenolic compound from watermelon seeds. Huachiew Chalermprakiet Science and Technology Journal, 7(2), 52-60.
Paran, E., Novack, V., Engelhard, Y. N., & Hazan-Halevy, I. (2009). The effects of natural antioxidants from tomato extract in treated but uncontrolled hypertensive patients. Cardiovascular Drugs and Therapy, 23, 145-151. doi:10.1007/s10557-008-6155-2
Pichairat, D., & Mahea, N. (2014). Effect of blanching on the phenolic content and antioxidant capacities of some Southern Thai indigenous vegetables. Rajamangala University of Technology Srivijaya Research, 6(2), 36-46.
Pranprawit, A. (2019). Local vegetables traditionally used for lowering hyperglycemia in Surat Thani Province, Thailand. International Journal of Applied Pharmaceutics, 11(S3), 3-6.
doi:10.22159/ijap.2019.v11s3.M0008
Pranprawit, A., Heyes, J. A., Molan, A. L., & Kruger, M. C. (2015). Antioxidant activity and inhibitory potential of blueberry extracts against key enzymes relevant for hyperglycemia. Journal of Food Biochemistry, 39(1), 109-118. doi:10.1111/jfbc.12094
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10), 4290-4302. doi:10.1021/jf0502698
Sarker, U., & Oba, S. (2020). Nutrients, minerals, pigments, phytochemicals, and radical scavenging activity in Amaranthus blitum leafy vegetables. Scientific Reports, 10, 3868. doi:10.1038/s41598-020-59848-w
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
doi:10.1016/S0076-6879(99)99017-1
Thonginla, V., Wanwimolruk, C., & Chuaybamroong, P. (2014). Correlation between antioxidant capacities of fruits analyzed with DMPD and phenolic contents, vitamin C, vitamin E and beta-carotene. Burapha Science Journal, 19(2), 93-104.
United States Environmental Protection Agency. (1996). Method 3052: Microwave assisted acid digestion of siliceous and organically based matrices. Retrieved from https://www.epa.gov/sites/default/files/2015-12/documents/3052.pdf
Waterhouse, A. L. (2002). Determination of total phenolics. Current Protocols in Food Analytical Chemistry. New York: John Wiley & Sons.
Yakoh, K., Weschasat, T., & Suwannachote, P. (2017). Antioxidant activity of indigenous vegetables in Suratthani Province. Proceeding of National & International Conference: Research 4.0 innovation and development SSRU’s 80th anniversary (pp. 233-243). Bangkok, Thailand.
Yakoh, K., Weschasat, T., & Suwannachote, P. (2018). Phenolic compound of 10 indigenous vegetables from Surat Thani Province. Journal of Thai Traditional and Alternative Medicine, 16(2), 185-194.
Zehiroglu, C., & Sarikaya, S. B. O. (2019). The importance of antioxidants and place in today’s scientific and technological studies. Journal of Food Science and Technology, 56, 4757-4774.
doi:10.1007/s13197-019-03952-x
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