Biotechnological Potential of Marine-Derived Fungi for  Textile Dye Degradation via Laccase-Like Activity

Ugochukwu   Okechukwu Ozojiofor; Mohammed  Sani  Abdulsalami; Nkechi Eucharia  Egbe; Ahmed Ali  Haroun; Israel Olufemi  Adedeji; Sherifat Bukola Ayuba-Buhari Ayuba-Buhari

doi:10.53848/ssstj.v12i2.974

Authors

Ugochukwu Okechukwu Ozojiofor Nigerian Defence Academy, Kaduna, Nigeria
Mohammed Sani Abdulsalami Nigerian Defence Academy, Kaduna, Nigeria
Nkechi Eucharia Egbe Nigerian Defence Academy, Kaduna, Nigeria
Ahmed Ali Haroun Nigerian Defence Academy, Kaduna, Nigeria
Israel Olufemi Adedeji University of Lagos, Idi-araba, Lagos
Sherifat Bukola Ayuba-Buhari Ayuba-Buhari Nigerian Defence Academy, Kaduna, Nigeria

DOI:

https://doi.org/10.53848/ssstj.v12i2.974

Keywords:

Meyerozyma guilliermondii, Penicillium oxalicum, Marine fungi, Laccase-like activity, Dyes, Oxidative

Abstract

The complex structure of synthetic dyes has made them challenging to decolorize, despite their widespread use in a variety of industries, including textiles, cosmetics, printing, paper, and pharmaceuticals. Enzymes such as oxidoreductases and peroxidases are employed in novel biotechnological procedures. An affordable and environmentally friendly solution to the issue of decolorizing commercial dyes is the use of oxidative enzymes, such as laccases derived from fungi. This study screened marine-derived fungal strains isolated from five coastal areas in Lagos, Nigeria, in order to identify laccase-like activities and assess the isolates' ability to decolorize industrial dyes in solid and liquid media by monitoring the radial growth, percentage decolorization, biosorption, and laccase activity after seven days of incubation with the dyes. After several steps of culturing on potato dextrose agar (PDA) plates, forty pure fungal cultures were isolated from a total of 100 samples (wood, nets, plants, clothing materials, soil, and water) collected from five different marine biotopes in Lagos: Oniru Beach, Makoko Lagoon, Elegushi Beach, Adekunle Lagoon, and Unilag waterfront. Through preliminary oxidative ability screening with 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), eleven isolates were discovered. Six strains of Meyerozyma guilliermondii, two strains of Rhodotorula mucilaginosa, one strain of Candida tropicalis, and two strains of Penicillium oxalicum were identified as belonging to the following species by means of molecular-based taxonomic approaches. Meyerozyma guilliermondi A4B and Penicillium oxalicum M6A effectively decolorized four synthetic dyes from various dye families and demonstrated the highest level of oxidative capacity and grew best at 2 mM CuSO₄ as an inducer and 2% and 1% NaCl to mimic a marine environment, and both produced the highest laccase-like active cell-free supernatants activity.

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