การทดสอบสารพฤกษเคมีเบื้องต้นและฤทธิ์ต้านออกซิเดชันของมันเสา (Dioscorea alata L.): Preliminary Phytochemical Screening and Antioxidant Activity of Dioscorea alata L.

Phukphon Munglue; Khwanduean Rattana; Supavee Sangchanjiradet; Nualyai  Yaraksa; Supannee Aoki

Authors

Phukphon Munglue Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Khwanduean Rattana Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Supavee Sangchanjiradet Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Nualyai Yaraksa Program of Chemistry, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Supannee Aoki Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani

Keywords:

Dioscorea alata L., Phytochemicals, Flavonoid content, Total phenolic content, Antioxidant activity

Abstract

The aim of this research was to evaluate the preliminary phytochemical screening and antioxidant activity of Dioscorea alata L.. The samples of dried D. alata peel and flesh powders were extracted using 80% ethanol or methanol. The phytochemical study of the extracts showed the presence of phenols, flavonoids, terpenoids, anthraquinones, tannins and glycosides. The highest total phenolic content was found in the methanolic D. alata flesh extract (580.49±0.91 mg GAE/g of extract) (P<0.05). The methanolic D. alata peel extract showed the highest value of total flavonoid content (339.86±1.36 mg QE/ g of extract) (P<0.05). The ethanolic D. alata flesh extract exhibited the maximum antioxidant activity in DPPH assay (IC₅₀ = 13.09±0.12 mg/L) when compared with ascorbic acid (IC₅₀ = 11.19±1.92 mg/L) (P>0.05). The results also indicated that methanolic D. alata peel (IC₅₀ = 3.10±0.13 mg/L) and flesh (IC₅₀ = 4.35±0.18 mg/L) extracts had high antioxidant activity in the ABTS assay and did not differ when compared with Trolox® (IC₅₀ = 4.38±0.65 mg/L) (P>0.05). ß-carotene content of D. alata peel (30.10±0.09 µg/100 g fresh weight) was significantly higher than that of the flesh (7.21±0.05 µg/100 g fresh weight) (P<0.05). D. alata flesh (43.46±2.24 mg/L) showed significantly lower in the value of anthocyanin content as compared to the peel (58.05±3.75 mg/L) (P<0.05). This study indicated that D. alata composted with several phytochemicals, phenolics and flavonoids possess antioxidant activity and could be useful for the application in food ingredients, cosmetics and pharmaceuticals.

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Preliminary Phytochemical Screening and Antioxidant Activity of Dioscorea alata L.