การทดสอบสารพฤกษเคมีเบื้องต้นและฤทธิ์ต้านออกซิเดชันของมันเสา (Dioscorea alata L.): Preliminary Phytochemical Screening and Antioxidant Activity of Dioscorea alata L.

Phukphon Munglue; Khwanduean Rattana; Supavee Sangchanjiradet; Nualyai  Yaraksa; Supannee Aoki

Authors

Phukphon Munglue Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Khwanduean Rattana Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Supavee Sangchanjiradet Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Nualyai Yaraksa Program of Chemistry, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani
Supannee Aoki Program of Biology, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubon Ratchathani

Keywords:

Dioscorea alata L., Phytochemicals, Flavonoid content, Total phenolic content, Antioxidant activity

Abstract

The aim of this research was to evaluate the preliminary phytochemical screening and antioxidant activity of Dioscorea alata L.. The samples of dried D. alata peel and flesh powders were extracted using 80% ethanol or methanol. The phytochemical study of the extracts showed the presence of phenols, flavonoids, terpenoids, anthraquinones, tannins and glycosides. The highest total phenolic content was found in the methanolic D. alata flesh extract (580.49±0.91 mg GAE/g of extract) (P<0.05). The methanolic D. alata peel extract showed the highest value of total flavonoid content (339.86±1.36 mg QE/ g of extract) (P<0.05). The ethanolic D. alata flesh extract exhibited the maximum antioxidant activity in DPPH assay (IC₅₀ = 13.09±0.12 mg/L) when compared with ascorbic acid (IC₅₀ = 11.19±1.92 mg/L) (P>0.05). The results also indicated that methanolic D. alata peel (IC₅₀ = 3.10±0.13 mg/L) and flesh (IC₅₀ = 4.35±0.18 mg/L) extracts had high antioxidant activity in the ABTS assay and did not differ when compared with Trolox® (IC₅₀ = 4.38±0.65 mg/L) (P>0.05). ß-carotene content of D. alata peel (30.10±0.09 µg/100 g fresh weight) was significantly higher than that of the flesh (7.21±0.05 µg/100 g fresh weight) (P<0.05). D. alata flesh (43.46±2.24 mg/L) showed significantly lower in the value of anthocyanin content as compared to the peel (58.05±3.75 mg/L) (P<0.05). This study indicated that D. alata composted with several phytochemicals, phenolics and flavonoids possess antioxidant activity and could be useful for the application in food ingredients, cosmetics and pharmaceuticals.

References

Evans P, Halliwell B. Micronutrients: oxidant/antioxidant status. Br J Nutr 2001;85(S2):S67–74.

ศิริธร ศิริอมรพรรณ. สารต้านอนุมูลอิสระในอาหาร. กรุงเทพฯ: สำนักพิมพ์โอเดียน สโตร์; 2557.

Burton GW, Ingold KU. ß-carotene: an unusual type of lipid antioxidant. Science 1984;224(4649):569-73.

Madhavi DL, Deshpande SS, Salunkhe DK. Toxicological aspects of food antioxidants. In: Food antioxidants. New York: Marcel Dekker Inc; 1995.

รวินิภา ศรีมูล. สารประกอบฟีนอลิกและฤทธิ์ต้านออกซิเดชันในพืช. กรุงเทพฯ: โอ. เอส. พริ้นติ้ง เฮ้าส์; 2563.

Price EJ, Wilkin P, Sarasan V, Fraser PD. Metabolite profiling of Dioscorea (yam) species reveals under utilised biodiversity and renewable sources for high-value compounds. Sci Rep 2016;6:29136.

Padhan B, Panda D. Potential of neglected and underutilized yams (Dioscorea spp.) for improving nutritional security and health benefits. Front Pharmaco 2020;11:496.

Maithili V, Dhanabal SP, Mahendran S, Vadivelan R. Antidiabetic activity of ethanolic extract of tubers of Dioscorea alata in alloxan induced diabetic rats. Indian J Pharmacol 2011;43(4):455-59.

สมนึก พรมแดง, รงรอง หอมหวล, มณฑา วงศ์มณีโรจน์, รัตนา เอการัมย์ และสุลักษณ์ แจ่มจำรัส. สารสำคัญทางโภชนาการของมันเลือด. วารสารวิชาการเกษตรศาสตร์ กำแพงแสน 2561;1(1):19-27.

Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Sci World J 2017;5873648.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16(3):144-58.

Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 2012;12:221.

Yaraksa N. Antioxidant activity of Markhamia stipulata, Caryota maxima, Amphineurion marginatum extracts and cytotoxicity effect on human dermal fibroblast cells. RMUTI J Sci Technol 2019;12(2):138-48.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolourization assay. Free Radic Biol Med 1999;26(9-10):1231-37.

Nour V, Trandafir I, Ionica ME. Antioxidant compounds, mineral content and antioxidant activity of several tomato cultivars grown in Southwestern Romania. Not Bot Horti Agrobo 2013;41(1):136-42.

Shao Y, Xu F, Sun X, Bao J, Beta T. Phenolic acids, anthocyanins and antioxidant capacity in rice (Oryza sativa L.) grains at four stages of developments after flowering. Food Chem 2014;143:90-6.

Saxena M, Saxena J, Nema R, Singh D, Gupta A. Phytochemistry of medicinal plants. J Pharmacogn Phytochem 2013;1(6):168-82.

Guo X, Sha X, Cai S, Wang O, Ji B. Antiglycative and antioxidative properties of ethyl acetate fraction of Chinese Purple Yam (Dioscorea alata L.) extracts. Food Sci Technol Res 2015;21(4):563-71.

Anisuzzman M, Zilani MNH, Khushi SS, Asaduzzman M. Antioxidant, antibacterial potential and HPLC analysis of Dioscorea alata Bulb. Indones J Pharm 2016;27(1):9-14.

Kwon JB, Kim MS, Sohn HY. Evaluation of antimicrobial, antioxidant, and antithrombin activities of the rhizome of various Dioscorea species. Korean J Food Preserv 2010;17(3):391-97.

Freeman BL, Eggett DL, Parker TL. Synergistic and antagonistic interactions of phenolic compounds found in navel oranges. J Food Sci 2010;75(6):C570-76.

Landi M, Tattini M, Gould KS. Multiple functional roles of anthocyanins in plant-environment interactions. Environ Exp Bot 2015;119:4–17.

Mattioli R, Francioso A, Mosca L, Silva P. Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules 2020;25(17):3809.

Preliminary Phytochemical Screening and Antioxidant Activity of Dioscorea alata L.