Effects of Liquid Vermicompost on Growth of Jewel Orchid in Tissue culture

ผลของน้ำหมักมูลไส้เดือนดินต่อการเจริญเติบโตของว่านน้ำทองในสภาพปลอดเชื้อ

Abstract

          The objective of this study the effect of liquid vermicompost on growth of Jewel Orchid in tissue culture. The node of Jewel Orchid was cultured on modified MS media using liquid vermicompost at 4 concentrations, 15, 20, 25, and 30 ml/l, instead of the MS stock 1 (NH₄NO₃, KNO₃, KH₂PO₄, H₃BO₃, KI), with coconut water 150 ml/l and BA 0.2 mg/l compared with the Murashige and Skoog (MS) medium which were supplemented 150 ml/l of coconut water and 0.2 mg/l of BA.  The cultures were incubated at temperature 25±2 ^oC with light 16 hrs./day for 60 days. The results showed that media using liquid vermicompost at 15 ml/l with 150 ml/l of coconut water and 0.2 mg/l of BA gave the highest number of shoots, plant height, number of leaves, leaf width, number of root and root length (2.60±0.52 shoots 17.3±0.31 mm. 2.70±0.48 leaves 9.1±0.22 mm. 2.60±0.69 roots 6.7±0.17 mm. respective) had significant differences (P<0.05) and gave growth bester more than MS media. Therefore, liquid vermicompost trend can be used as a replacement for MS stock 1 in tissue culture.