ผลของไคเนตินที่มีต่อการชักนำให้เพิ่มจำนวนยอดของเมล็ดมะกรูด (Citrus hystrix DC.) โดยการเพาะเลี้ยงเนื้อเยื่อ: The Effect of Kinetin on Multiple Shoot Induction of  Kaffir Lime Seeds (Citrus hystrix DC.) by Tissue Culture

Phonthip Terdbaramee; Kanitta Onsiri

Authors

Phonthip Terdbaramee General Science, Faculty of Science, Chandrakasem Rajabhat University, Bangkok
Kanitta Onsiri General Science, Faculty of Science, Chandrakasem Rajabhat University, Bangkok

Keywords:

Citrus hystrix DC. , Plant tissue culture, Kinetin

Abstract

The aim of this study was used to investigate the effect of Kinetin (KIN) on multiple shoot induction of Kaffir lime (Citrus hystrix DC.) seeds from cultivation on Murashige and Skoog (MS) medium supplemented with KIN at various concentrations of 0, 1, 2 and 3 mg/L for 4 weeks. After 4 weeks of the Kaffir lime seeds cultivation, the MS medium supplemented with KIN at the concentration of 1, 2, and 3 mg/L produced the highest average number of the induced shoot which is 100 %. In contrast, MS medium without KIN was 85.71%. Additionally, it was found that the MS medium containing KIN at the concentration of 1 mg/L was able to induce the highest average number of shoots to 2.86±1.07 shoots per seed and the percentage of root formation was 100%.

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References

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The Effect of Kinetin on Multiple Shoot Induction of Kaffir Lime Seeds (Citrus hystrix DC.) by Tissue Culture