ผลของไคเนตินที่มีต่อการชักนำให้เพิ่มจำนวนยอด ของเมล็ดมะกรูด (Citrus hystrix DC.) โดยการเพาะเลี้ยงเนื้อเยื่อ: The Effect of Kinetin on Multiple Shoot Induction of  Kaffir Lime Seeds (Citrus hystrix DC.) by Tissue Culture

Phonthip Terdbaramee; Kanitta Onsiri

Authors

Phonthip Terdbaramee General Science, Faculty of Science, Chandrakasem Rajabhat University
Kanitta Onsiri General Science, Faculty of Science, Chandrakasem Rajabhat University

Keywords:

Citrus hystrix DC. , Plant tissue culture, Kinetin

Abstract

Tissue culture of Kaffir lime (Citrus hystrix DC.) seeds was used to study the effect of Kinetin on multiple shoot induction from cultivation on Murashige and Skoog (MS) medium supplemented with Kinetin at various concentrations of 0, 1, 2 and 3 mg/L. after 4 weeks cultivation of the Kaffir lime seeds, MS medium supplemented with the concentration of 1, 2, 3 mg/L of KIN produced the highest average number of induced shoot which is equal 100 %. MS medium with KIN added Not added. KIN originated from the production of kaffir lime seeds that arrived at 85.71and when studying the induction of increasing the number of shoots. It was found that the MS medium containing KIN at the concentration of 1 mg/l The highest average number of shoots was 2.86±1.07 shoots per seed (mean shoots: 3.20±0.67 shoots) and the percentage of root formation was 100%.

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The Effect of Kinetin on Multiple Shoot Induction of Kaffir Lime Seeds (Citrus hystrix DC.) by Tissue Culture