สารสกัดเอทานอลจากรำข้าวเหนียวแดง (Oryza sativa L.)  ลดภาวะ oxidative stress ในเซลล์มะเร็งตับ HuH-7: Ethanolic extract of bran of red sticky rice (Oryza sativa L.)  attenuates cellular oxidative stress in human hepatocellular  carcinoma cell line (HuH-7 cells)

Atthapan  Morchang; Shilu Malakar; Patsarawadee Paojinda; Narongsuk Munkong; Kavalin Vongthoung

Authors

Atthapan Morchang Medical Technology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok
Shilu Malakar Department of Molecular Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok
Patsarawadee Paojinda Medical Technology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok
Narongsuk Munkong Department of Pathology, School of Medicine, University of Phayao, Phayao
Kavalin Vongthoung Medical Technology Program, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok

Keywords:

Red sticky rice bran extract, Oxidative stress, ROS, Glutathione

Abstract

Oxidative stress is an imbalance event between free radical accumulation and antioxidant levels within the cells. It is the major cause of several chronic diseases. Red sticky rice is the pigmented rice, which being commonly cultivated and consumed in Thailand. Our previous study demonstrated that an extract from red sticky rice bran (RRBE) contains plenty of antioxidant substances. Thus, this research aimed to investigate the effect of RRBE against oxidative stress within the cells. The results showed that RRBE increased the cell survival rate of HuH-7 cells upon insulted with oxidative stress inducer, H₂O₂. Moreover, RRBE decreased the ROS production while increased glutathione production within the cells. Altogether, our results demonstrated the effect of RRBE against cellular oxidative stress proven its potential use as antioxidant supplement.

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Ethanolic extract of bran of red sticky rice (Oryza sativa L.) attenuates cellular oxidative stress in human hepatocellular carcinoma cell line (HuH-7 cells)