Acute and Chronic Toxicity of Battery Waste Leachates to Daphnia magna


  • Van-Tai Nguyen Ho Chi Minh City University of Technology, VNU-HCM, Vietnam
  • The-Ton Phan Ho Chi Minh City University of Technology, VNU-HCM, Vietnam
  • Thi-My-Chi Vo Ho Chi Minh City University of Technology, VNU-HCM, Vietnam
  • Thanh-Luu Pham Institute of Tropical Biology, Vietnam Academy of Science and Technology, Vietnam
  • Manh-Ha Bui Department of Environmental Sciences, Saigon University, Vietnam
  • Thanh-Son Dao Ho Chi Minh City University of Technology, VNU-HCM, Vietnam


Negative effects, Battery leachates, Life history traits, Daphnia magna


In developing countries, e-waste battery treatment and management is a severe problem. Most batteries contain heavy metals, but some can contain very toxic heavy metals such as mercury that can be hazardous to the environment. The current investigation aims to evaluate both the acute and chronic effects of the leachates from two cells (named Con O and Maxell) that are commonly used in Vietnam on the micro-crustacean Daphnia magna. The median lethal concentrations at 24h and 48h (LC50) of the D. magna exposed to leachate from Con O cell were 150 and 100 mg/l, respectively. The 24h- and 48h-LC50 values of the Maxell cell to the animals
were 100 and 70 mg/l, respectively. Therefore, the toxicity of the leachates from the Maxell battery was more severe than that of Con O based on the 24h- and 48h-LC50 values. Moreover, the life-history traits of the D. magna such as survivorship, maturation or reproduction, were detrimentally impacted including mass mortality, delayed maturation and reproductive inhibition, when the animals exposed to the leachates from both batteries at the concentrations ranged from 1 to 50 mg/l over the period of 2 weeks. Overall, this study could provide useful information on the ecological and environmental risk caused by untreated batteries to the environment, or even contribute to changing a sense of civic responsibility on economically using, recycling, waste management and
treatment related to cells.


Aaltonen, M., Peng, C., Wilson, B. P., & Lundstrom, M. (2017). Leaching of metals from spent lithium-ion batteries. Recycling, 2,20.

Adema, D. M. M. (1978). Daphnia magna as a test animal in acute and chronic toxicity tests. Hydrobiologia, 59(2), 125-134.

American Public Health Association. (2012).Standard methods for the examination of water and wastewater (22nd ed.). Washington, DC: American Water Works Association, Water Environment Federation.

Araujo, G. S., Pavlaki, M. D., Soares, A. M. V. M., Abessa, D. M. S., & Loureiro, S. (2019). Bioaccumulation and morphological traits in a

multi-generation test with two Daphnia species exposed to lead. Chemosphere, 219,636-644.

Barata, C., Baird, D. J., Nogueira, A. J. A., Soares, A. M. V. M., & Riva, M. C. (2006). Toxicity of binary mixture of metals and pyrethroid

insecticides to Daphnia magna Straus.Implications for multi-substance risks assessment. Aquatic Toxicology, 78, 1-14.

Biesinger, K. E., & Christensen, G. M. (1972). Effects of various metals on survival, growth, reproduction and metabolism of Daphnia

magna. Journal of the Fisheries Research Board of Canada, 29, 1691-1700.

Chu, K. W., & Chow, K. L. (2002). Synergistic toxicity of multiple heavy metals is revealed by a biological assay using a nematode and its

transgenic derivative. Aquatic Toxicology, 61,53-64.

Fisher, K., Wallén, E., Laenen, P. P., & Collins, M.(2006). Battery Waste Management Life Cycle Assessment. Environmental Resources

Management. Retrieved from

Frías-Espericueta, M. G, Castro-Longoria, R.,Barrón-Gallardo, G. J., Osuna-López, J. I.,Abad-Rosales, S. M., Páez-Osuna, F., &

Voltolina, D. (2008). Histological changes and survival of Litopenaeus vannamei juveniles with different copper concentrations.

Aquaculture, 278, 97-100.

Ghazy, M. M., & Habashy, M. M. (2003).Experimental toxicity of chromium to two freshwater crustaceans: Daphnia magna and

Macrobrachium rosenbergii. Egyptian Journal of Aquatic Biology and Fisheries, 7(3), 49-70.

Giraudo, M., Dube, M., Lepine, M., Gagnon, P., Douville, M., & Houde, M. (2017). Multigenerational effects evaluation of the flame retardant tris (2-butoxyethyl) phosphate (TBOEP) using Daphnia magna. Aquatic Toxicology, 190, 142-149.

Jho, E. H., An, J., & Nam, K. (2011). Extended biotic ligand model for predictions of mixture toxicity of Cd and Pb using single metal

toxicity data. Environmental Toxicology and Chemistry, 30(7), 1697-1703.

Kim, H. Y., Lee, C. K., Lee, J. T., Moon, C. S., Ha, S. C., Kang, S. G., ... Kang, M. G. (2009).Effects of manganese exposure on dopamine

and prolactin production in rat. Neuroreport,20(1), 69-73.

Kooijman, S. A. L. M. (2000). Dynamic energy and mass budgets in biological systems (2nd ed.).Cambridge, United Kingdom: Cambridge

University Press.

LeBlanc, G. A. (1982). Laboratory investigation into the development of resistance of Daphnia magna (Straus) to environmental pollutants.

Environmental Pollution Series A, 27(4), 309-322.

Luciana, R., Ulises, R., Susana, G., Horacio, T., & Maria, G. A. (2014). Effect of metals on Daphnia magna and cladocerans

representatives of the Argentinean Fluvial Littoral. Journal of Environmental Biology, 35(4), 689-697.

Meshram, P., Abhilash, Pandey, B. D., Mankhand, T. R., & Deveci, H. (2016). Acid baking of spent lithium ion batteries for selective

recovery of major metals: A two-step process. Journal of Industrial and Engineering Chemistry, 43, 117-126.

Muyssen, B. T. A., De Schamphelaere, K. A. C., & Janssen, C. R. (2006). Mechanisms of chronic waterborne Zn toxicity in Daphnia magna.

Aquatic Toxicology, 77(4), 393-401.

Nguyen, T. D., Vo, T. M. C, Dao, C. T., Quang, N. X., & Dao, T. S. (2016). Chronic effects of industrial wastewater on life history traits of

Daphnia magna under the laboratory conditions. International Journal of Agriculture and Environmental Research, 2(4), 1000-1012.

Okamoto, A., Yamamuro, M., & Tatarazako, N. (2015). Acute toxicity of 50 metals to Daphnia magna. Journal of Applied

Toxicology, 35(7), 824-830.

Peiro, L. T., Mendez, G. V., & Ayres, R. U. (2013). Lithium: Sources, production, uses, and recovery outlook. The Journal of The

Minerals, Metals & Materials Society, 65(8),986-996.

Ramirez, D. C. S. (2014). Tolerance of a metal adapted natural Daphnia magna population to new stressors (Master’s dissertation). Ghent

University, Ghent, Belgium.

Rathore, R. S. (2001). Studies on the use of some freshwater invertebrates as sensitive test models for the assessment of toxicity of

environmental pollutants (Doctoral dissertation). University of Lucknow, Lucknow, Uttar Pradesh, India.

Shaw, J. R., Dempsey, T. D., Chen, C. Y., Hamilton, J. W., & Folt, C. L. (2006).Comparative toxicity of cadmium, zinc, and

mixtures of cadmium and zinc to daphnids.Environmental Toxicology and Chemistry,25(1), 182-189.

Sterner, R. W. (2009). Role of zooplankton in aquatic ecosystems. In Encyclopedia of inland waters (pp. 678-688). Oxford, UK: Elsevier.

Tran, P. T., Do-Hong, L. C., & Dao, T. S. (2014). Long-term impacts of copper and chromium on survivorship, maturation, fecundity and

growth of Daphnia magna. Vietnamese Journal of Science and Technology, 52, 309-315.

Traudt, E. M., Ranville, J. F., Smith, S. A., & Meyer, J. S. (2016). A test of the additivity of acute toxicity of binary-metal mixtures of Ni

with Cd, Cu, and Zn to Daphnia magna, using the inflection point of the concentration-response curves. Environmental Toxicology

and Chemistry, 35(7), 1843-1851.

U.S. Environmental Protection Agency. (2002). Methods for measuring the acute toxicity of effluents and receiving waters to freshwater

and marine organisms (5th ed.). Washington, DC: Author.

Versieren, L., Smets, E., De Schamphelaere, K., Blust, R., & Smolders, E. (2014). Mixture toxicity of copper and zinc to barley at low

level effects can be described by the Biotic Ligand Model. Plant and Soil, 381, 131-142.

Wagner, M., & Oehlmann, J. (2009). Endocrine disruptors in bottled mineral water: Total estrogenic burden and migration from plastic

bottles. Environmental Science and Pollution Research, 16(3), 278-286.

Yasuda, K., & Tanaka, M. (2006). Report on hazardous household waste generation in Japan. Waste Management and Research, 24,397-401.




How to Cite

Nguyen, V.-T. ., Phan, T.-T. ., Vo, . T.-M.-C. ., Pham, T.-L., Bui, M.-H. ., & Dao, T.-S. . (2022). Acute and Chronic Toxicity of Battery Waste Leachates to Daphnia magna. Suan Sunandha Science and Technology Journal, 7(2), 37–43. Retrieved from



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