Optimal Growth Conditions of Auricularia cornea Mycelium Isolated from Para Rubber Plantation
DOI:
https://doi.org/10.57260/stc.2026.1465คำสำคัญ:
Optimal conditions, Growth factor, Para rubber plantation, Auricularia corneaบทคัดย่อ
A survey of para rubber plantations belonging to farmers in Wang Takien Subdistrict, Khao Saming District, Trat Province, Thailand, revealed the presence of edible wood ear mushrooms (Auricularia cornea). Pure cultures were subsequently isolated from the mycelium of this mushroom and identified using molecular identification. The strains were then preserved in the laboratory for further research. The objective of this research was to determine the factors affecting the growth of the mycelium of A. cornea isolated from para rubber plantation. The factors, glucose concentration (2, 2.5, 3, 3.5, 4, 4.5, and 5%), pH (4, 5, 6, 7, 8, and 9), and temperature (25, 30, 35, and 40ºC) had been investigated on mycelium growth. The factorial completely randomized design (Factorial CRD) was used to design all the experiments. It was found that this strain grew the greatest on Potato Dextrose Agar (PDA) containing 2% glucose, which had the significantly highest colony diameter (7.51±0.21 cm at 8 days, p-value<0.05). At pH 6 and 7, the mycelium grew similarly well and condensed mycelium that revealed colonies diameter of 7.55±0.57 and 7.79±0.47 cm at 10 days, respectively (not significantly different). Finally, the mycelium was able to grow the best at 30ºC with the significantly highest colony diameter (8.03±0.89 cm at 6 days, p-value<0.05). The findings of this study revealed that the optimal conditions for A. cornea mycelium cultivation are growth on PDA containing 2% glucose, at pH 6 or 7, and incubation at 30ºC. Under these optimal conditions, the mycelium of this strain grew rapidly and exhibited dense growth. Therefore, it is suitable for application in cultivating high-quality mycelium before fruiting body cultivation.
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