Selection of carbaryl and cypermethrin-specific DNA aptamers for quantitative detection using electrochemical aptasensor technique
DOI:
https://doi.org/10.55164/jtai.v3i2.1229Keywords:
DNA aptamer, Carbaryl, Cypermethrin, Pesticides, BiosensorAbstract
Testing for pesticide residue is a necessary step in the food safety production process. However, testing is complicated, time-consuming, and costly. Therefore, this research aimed to develop a simple and rapid method for pesticide residue detection. This study aimed to select DNA aptamers specific to carbaryl and cypermethrin for the development of an electrochemical aptasensor-based detection system. Carbaryl and cypermethrin standards were used as aptagen targets during aptamer selection via Systematic Evolution of Ligands by Exponential enrichment (SELEX) and indirect enzyme-linked aptamer assay(ELAA). A total of 13 and 22 DNA aptamer strands binding to carbaryl and cypermethrin, respectively, were successfully isolated. Among these, the carbaryl-binding aptamer Car26 and cypermethrin-binding aptamer Cyp126 were immobilized onto screen-printed gold electrodes (SPE gold) to construct biosensors for detecting pesticide residues in vegetables. The developed aptasensors demonstrated low detection limits (LOD) of 0.049 ppm for carbaryl and 0.044 ppm for cypermethrin. Compared to conventional chemical analysis methods, this electrochemical aptasensor offers lower cost, digital quantitative output, and user-friendly operation, making it a practical tool for rapid on-site screening.
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