Effects of BA and NAA on growth and development of tissue sweet potato cultivars “Luang Sai Nam-Phuong Indo” in vitro

Abstract

This study aimed to determine the effect of BA (6-benzyl adenine) and NAA (1-Naphthalene acetic) concentrations on growth and development and root induction of tissue sweet potato cultivars “Luang Sai Nam-Phuong Indo” in vitro. Two experiments; 1^st experiment studied on concentrations of BA (0.5, 1 and 2 mg/l) in combination with NAA (0.1, 0.3, and 0.5 mg/l) used factorial in completely randomized design (CRD) 10 treatment, ten repetitions. 2^nd Experiment studied on NAA concentrations (0.1, 0.3 and 0.5 mg/l) added to the cutting-balls compared to not NAA in the cutting-balls and cultured on a semi-solid MS medium, planned complete randomized design (CRD), five treatments with ten replications. After 4 weeks, the result showed that, in experiment 1, the contamination of all treatments was no different (about 10%). In the medium, add 0.50 and 1.00 mg/l BA combination with 0.10 mg/l NAA (fresh and dry weight of callus) height growth and development of callus, but control medium gave the greatest root length (1.49±0.21 and 7.47±1.75 cm, respectively). The root induction of the shoot tip was not and added with NAA in the cutting-ball was no different with culture on semi-soil MS medium. Except for root length (cm), it was the greatest when the shoot tip cultured on semi-soil MS medium was 8.47±1.46 cm.