Cultivation of the seagrass Thalassia hemprichii (Ehrenberg) Ascheron

Abstract

Study on tissue culture of turtle sesame Thalassia hemprichii (Ehrenberg) Ascherson. From the study of bleaching and sterilizing, In formulas 1-4, high-tech washing at level 20, cost-effective 5,10,15 and 20 minutes, and formula 5-7, high-tech washing at level 1, at level Intensive 8, 10 and 12 percent, peer 10 minutes, and Level 2 at levels 4, 5 and 6, peer 15 minutes. found that bleaching and sterilization the most suitable method was method 3, which was sterilized with 20 percent haiter for 15 min. Survival was from leaf parts 26.67 percent (8.00±0.41), leaf base parts with 23.33 percent (7.00±0.44) and survival and stem fragments with 21.10 percent survival (6.33±0.50) It was found that bleaching with haiter at 20 percent concentration for 15 minutes was the most effective method. is having the lowest microbial contamination and from the study of growth regulators that are suitable for tissue development of turtle leaches (Thalassia hemprichii) (Ehrenberg) Ascherson at various concentrations of BA (6-Benzyl aminopurine)             at concentrations of 0, 0.5, 1.0, 2.0 and 3.0 mg/l and NAA (Naphthalene acetic acid) concentration levels 0 0.5 1.0 2.0 and 3.0 mg/l Within 2 months, the percentage of leaf fragment survival At various concentrations of BA at a concentration of 2.0 mg/l and NAA at a concentration of 2.0 mg/l, 0.77 percent (0.23±0.40) percent survival of leaf base parts. at various concentrations of BA at a concentration of 2.0 mg/l. and NAA at concentrations of 2.0 mg/l, 0.80 percent (0.24±0.31) and percentage survival of underground stem fragments. at various concentrations of BA at a concentration of 2.0 mg/l and NAA at a concentration of 2.0 mg/l at 0.87 percent (0.26±0.33) and a growth of 1 new plant.