Spectrophotometric Determination of Chlorophyll and Carotenoid Contents of Amorphophallus muelleri Blume Leaves using Different Solvents
Keywords:
Amorphophallus muelleri, pigment, leaf greeness value, chlorophyll meterAbstract
The objectives of this study were to compare the chlorophyll and carotenoid content extracted from Amorphophallus. muelleri leaf tissues by different solvents and to evaluate the relationship between the extracted chlorophyll content and greenness values measured by SPAD meter. In the present study, chlorophyll and carotenoid were extracted from mature leaves by using dimethyl sulfoxide (DMSO), 95% ethanol, 80% acetone, and 90% acetone. The experiment was carried out with a completely randomized design with 30 replications (plants). It showed that the highest chlorophyll a, chlorophyll b and total chlorophyll content were observed when DMSO and 95%ethanol were used as extracted solvent. While the highest carotenoid concentration was obtained with 80% and 90% acetone solvent. SPAD reading and chlorophyll content were positively related. The linear regression equations obtained were y=0.0682x-1.0577 (R2 = 0.70), y=0.0459x-0.6321 (R2 = 0.67) and y=0.0221x-0.4285 (R2 = 0.73) to assess total chlorophyll, chlorophyll a and chlorophyll b content, respectively. It indicated that measurement by chlorophyll meter is a rapid technique for the evaluation of total chlorophyll in A. muelleri leaves
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