Preliminary Research on Sample Preparation Methods for Cannabinoid Content in Hemp Leaf
Keywords:
Agricultural residue, Completely Randomized Design, Hemp, TetrahydrocannabinolAbstract
A significant amount of leaf residue is produced during the commercial production of industrial hemp (Cannabis sativa L.). Farmers frequently burn the leaf because it is convenient and inexpensive. Additionally, residual cannabinoids limit their prospective use. This study aimed to investigate the effects of temperature and duration on the changes in cannabinoid content in hemp leaf residue treated with a hot air oven. A factorial experiment was conducted using a Completely Randomized Design (CRD) at temperatures of 70, 80, 85, 90, 100, and 110°C were applied for durations of 1, 2, 3, 4, 5, and 6 hours. Delta9–tetrahydrocannabinol (Δ9–THC) and Cannabidiol (CBD) content were analyzed using High Performance Liquid Chromatography (HPLC). The results showed that both temperature and duration significantly affected cannabinoid levels (P<0.05). Treatment at 110°C for 6 hours significantly reduced Δ9–THC content from 0.685% (w/w) to 0.442% (w/w), and Treatment at 90°C for 6 hours significantly increased CBD content from 0.622% (w/w) to 1.156% (w/w)
References
ประกาศกระทรวงสาธารณสุข. 2564.เรื่อง มาตรฐานการให้บริการของสถานพยาบาลโดยใช้ระบบบริการการแพทย์ทางไกล พ.ศ. 2564 ราชกิจจานุเบกษา 138 (ตอนพิเศษ 172 ง): 8.
ประกาศกระทรวงสาธารณสุข. 2565. เรื่อง ระบุชื่อยาเสพติดให้โทษในประเภท 5 พ.ศ. 2565ราชกิจจานุเบกษา 139 (ตอนพิเศษ 35 ง): 8.
สำนักงานเศรษฐกิจอุตสาหกรรม กระทรวงอุตสาหกรรม. 2566. แผนปฏิบัติการด้านการพัฒนาอุตสาหกรรมพืชกัญชงสู่เชิงพาณิชย์ (พ.ศ.2566– 2570). (ระบบออนไลน์). แหล่งข้อมูล: http://bioie.oie.go.th/oieqrcode/uploadFile/oie736117653_2029136235.pdf (4 เมษายน 2568).
Adesina, I., A. Bhowmik, H. Sharma and A. Shahbazi. 2020. A review on the current state of knowledge of growing conditions, agronomic soil health practices and utilities of hemp in the United States. Agriculture 10(4): 129.
Aizpurua-Olaizola, O., J. Omar, P. Navarro, M. Olivares, N. Etxebarria and A. Usobiaga. 2014. Identification and quantification of cannabinoids in Cannabis sativa L. plants by high performance liquid chromatography-mass spectrometry. Analytical and Bioanalytical Chemistry 406(29): 7549-7560.
Alegbeleye, O., O.A. Odeyemi, M. Strateva and D. Stratev. 2022. Microbial spoilage of vegetables, fruits and cereals. Applied Food Research 2(1): 100122.
Altman, A., C. Kent-Dennis, J. Klotz, K. McLeod, E. Vanzant and D. Harmon. 2024. Utilizing industrial hemp (Cannabis sativa L.) by-products in livestock rations. Animal Feed Science and Technology 307: 115850.
Chen, C., C. Venkitasamy, W. Zhang, L. Deng, X. Meng and Z. Pan. 2020. Effect of step-down temperature drying on energy consumption and product quality of walnuts. Journal of Food Engineering 285: 110105.
Chen, C., I. Wongso, D. Putnam, R. Khir and Z. Pan. 2021. Effect of hot air and infrared drying on the retention of cannabidiol and terpenes in industrial hemp (Cannabis sativa L.). Industrial Crops and Products 172: 114051.
Cheng, Y.C. and S. Kerrigan. 2024. Differentiation of hemp from marijuana using a qualitative decision-point assay. Forensic Chemistry 37: 100541.
García-Valverde, M.T., C. Sánchez-Carnerero Callado, M.C. Díaz-Liñán, V. Sánchez de Medina, J. Hidalgo-García, X. Nadal, L. Hanuš and C. Ferreiro-Vera. 2022. Effect of temperature in the degradation of cannabinoids: From a brief residence in the gas chromatography inlet port to a longer period in thermal treatments. Frontiers in Chemistry 10: 1038729.
Iffland, K., M. Carus and F. Grotenhermen. 2016. Decarboxylation of Tetrahydrocannabinolic acid (THCA) to active THC. (Online): Available Source: https://eiha.org/media/2014/08/16-10-25-Decarboxylation-of-THCA-to-active-THC.pdf (April 4, 2025).
Jaidee, W., I. Siridechakorn, S. Nessopa, V. Wisuitiprot, N. Chaiwangrach, K. Ingkaninan and N. Waranuch. 2022. Kinetics of CBD, Δ9-THC degradation and cannabinol formation in cannabis resin at various temperature and pH conditions. Cannabis and Cannabinoid Research 7(4): 537-547.
Kerdraon, L., V. Laval and F. Suffert. 2019. Microbiomes and pathogen survival in crop residues, an ecotone between plant and soil. Phytobiomes Journal 3(4): 246-255.
Knezevic, F., A. Nikolai, R. Marchart, S. Sosa, A. Tubaro and J. Novak. 2021. Residues of herbal hemp leaf teas – How much of the cannabinoids remain? Food Control 127: 101846.
McPartland, J.M., M. Duncan, V. Di Marzo and R.G. Pertwee. 2015. Are cannabidiol and Δ(9)-tetrahydrocannabivarin negative modulators of the endocannabinoid system? A systematic review. British Journal of Pharmacology 173(3): 737-753.
Poonsawat, T., N. Srilasak and I. Koodkaew. 2024. Allelopathic characterization and allelochemicals identification of hemp (Cannabis sativa L.) leaf residue. Industrial Crops and Products 222: 120003.
Radwan, M.M., S. Chandra, S. Gul and M.A. Elsohly. 2021. Cannabinoids, phenolics, terpenes and alkaloids of cannabis. Molecules 26(9): 2774.
Russo, E.B. and J. Marcu. 2017. Cannabis pharmacology: the usual suspects and a few promising leads. Advances in Pharmacology 80: 67-134.
Storm, C., M. Zumwalt and A. Macherone. 2019. A dedicated cannabinoid potency testing in cannabis or hemp products using the agilent 1220 infinity II LC system. (Online): Available Source: https://www.agilent.com/cs/library/applications/application-dedicated-cannabinoid-potency-testing-5991-9285-en-us-agilent.pdf (April 4, 2025).
Turturică, M., N. Stănciuc, G. Bahrim and G. Râpeanu. 2016. Effect of thermal treatment on phenolic compounds from plum (Prunus domestica) extracts–A kinetic study. Journal of Food Engineering 171: 200-207.
Wang, T., N. Wang, M. Wang, L. Wang, Y. Shi, J. Du and D. Yu. 2023. Theoretical exploration and experimental regulation of the degradation of Δ9-tetrahydrocannabinol in hemp seed oil by density functional theory. Food Research International 170: 112996.
White, K.H., D. Vergara, K.G. Keepers and N.C. Kane. 2016. The complete mitochondrial genome for Cannabis sativa. Mitochondrial DNA Part B 1(1): 715-716.
Woranisarakul, K. 2021. Pharmacology of Cannabinoids. Journal of Thai Stroke Society 20(3): 37-53.
Žugić, A., M. Martinović, V. Tadić, M. Rajković, G. Racić, I. Nešić and A. Koren. 2024. Comprehensive Insight into Cutaneous Application of Hemp. Pharmaceutics 16(6): 748.
