Genetic Diversity and Heterotic Grouping of Waxy Corn Inbred Lines Based on Sequence Related Amplified Polymorphism (SRAP) Markers
Keywords:
genetic diversity, heterotic, DNA marker, waxy cornAbstract
The success of the development of hybrid maize depends on the genetic differences of the inbred lines used as the parents. Therefore, the objective of this study was to assess the genetic diversity and heterotic grouping of 75 waxy corn samples including 62 waxy corn inbreds from National Corn and Sorghum Research Center (NCSRC), 8 commercial varieties and 5waxy corn samples from China with 16 SRAP markers. SRAP analysis revealed that the markers detected 199 alleles, of which 176 were polymorphic (88.44%). An average number of alleles per marker was 12.44. The average PIC value was 0.26 and gene diversity was 0.26. Neighbor-joining analysis and STRUCTURE analysis showed that all waxy corn samples were clustered into three heterotic groups. The first and second groups contained waxy corn inbreds from the NCSRC, while the third group had waxy corn inbreds from the NCSRC, commercial varieties and waxy corn from China. The findings in this study can be used for parental selection in order to breed a new waxy corn hybrid
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