The In Vitro Shoot Regeneration of Tobacco in Response to Light Spectra and Cytokinin
Keywords:
plant tissue culture, artificial light, plant growth and developmentAbstract
The effects of light spectra and plant growth regulator, BA, on tobacco tissue was conducted by incubated leaf blade and node explant obtained from tissue culture on solid MS media supplemented with 0, 1 and 2 mg/L BA. The cultures were incubated under cool white fluorescent and white, yellow, blue and red:blue LEDs for 4 weeks. The results demonstrated that BA inserted significant effect on shoot proliferation and medium containing 2 mg/L BA yielded the highest number of shoots. For the effect of light spectra, in the case of leaf blade explant, yellow LED yielded the highest number of shoots but not significant from white LED and fluorescent. The positive interaction between BA and light spectra was also observed. Similar results were observed in single node tissue explant but only the significant different in response of BA was observed and no interaction of BA and light spectra found.
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