In vitro Selection of Water-Deficit Tolerant Callus of Banana cv. ‘Kluai Hom Tong’
Keywords:
banana, Kluai Hom Tong, water-deficit tolerant, in vitroAbstract
The objective of this research was to select callus of banana ‘Kluai Hom Tong’ tolerated to water deficit by using polyethylene glycol (PEG) 6000 as a selective agent in aseptic conditions. The results showed that the MS medium containing 5 mg/L BA was suitable for inducing the highest number of shoots and initiated callus at the base of shoots. The 0.5 cm calli were cultured on MS medium containing 5 mg/L BA and 0 (control) 5, 10, 15 and 20% (w/v) of PEG for 2 months. It was found that MS medium containing BA 5 mg/L without PEG had an average shoot number of 5.1. Number of shoots derived from callus were decreased when they were cultured on MS media containing 5 -15% PEG. The color of the calli changed to black color and the lethal percentage was 50-60% in MS medium with 10 -15% PEG, while all calli changed to black at the level of 20% PEG . After calli were further cultured in the same media for one more month, the lethal percentage was increased. Then, all of the PEG-treated calli were transferred into MS medium containing 5 mg/L BA without PEG. The results showed that the new buds were induced from the survival callus derived from the media with 5-15% PEG, while none of the calli from 20% PEG developed into plantlet. In conclusion, the use of PEG at the level of 10-15% had a tendency to select the water-deficit tolerant callus of banana ‘Kluai Hom Tong’ at the laboratory level
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