Heat-Resistant Characteristic and Patulin Production Ability of Heat-Resistant Molds Isolated from Pineapple Field Soils
Keywords:
Heat-resistant mold, Ascospores, Patulin, Pineapple juice, Food safetyAbstract
Heat-resistant molds (HRMs) are a group of molds capable of producing ascospores that can withstand heat treatments at temperatures as high as 75°C for at least 30 min. The presence of HRM ascospores presents a significant challenge in the pasteurization of fruit juices. Moreover, some strains of HRMs can produce mycotoxins, which are harmful to consumers. This study aimed to characterize HRMs isolated from pineapple field soils in Chonburi and Kamphaeng Phet provinces, Thailand. The results revealed that HRMs were present in all soil samples. Subsequently, 60 representative isolates were randomly chosen to evaluate their ascospores-producing ability, which included 5 genera namely Aspergillus (with a neosartorya morph), Talaromyces, Penicillium (with an eupenicillium morph), Hamigera, and Paecilomyces (with a byssochlamys morph). Among these isolates, 28 isolates demonstrated the capacity to produce heat-resistant ascospores. Furthermore, this study observed patulin-producing capabilities in Paec. niveus and P. setosum strains, they produced patulin toxin in pineapple juice at higher concentrations, approximately 110 mg/mL. These findings emphasize the significance of the presence of these HRMs in pineapple field soils, as they can produce heat-resistant ascospores, and some strains are capable of producing patulin, which can lead to spoilage in pineapple and other fruit juices
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