Biological Activities of Phellinus sp.
Keywords:
Kratinphiman, Phellinus sp., Bioactive compound activityAbstract
In Thailand, the Kratinphiman Mushroom, Phellinus sp., has been reported in traditional Thai medicine with different species and common names. However, the identification of species in this genus is not clear; therefore, it is also not understood which one to use in the context of medicine or biologically active substance production. In this study, the active substances inhibited Bacillus subtilis and Streptococcus aureus were studied. The results revealed that the substances extracted from Phellinus sp. with ethyl-acetate could inhibit some strains of B. subtilis and S. aureuss. Phellinus linteus showed the highest inhibitory substance production when compared with streptomycin 400 ppm. Moreover, the separation of putative antibiotics by HPTLC and bio-autographic methods, demonstrated that the proportion of chloroform: methanol: acetic acid: H2O had a polarity value of 0.436. All Phellinus species produce low polarity antibiotics with an Rf value of 0.79. Moreover, P. noxius isolate PKD644 showed that the antibiotics could be clearly differentiated into two groups. Antibiotic production was from 7-36% from isolated biological compounds. The results revealed that isolate PKD644 produced the most antibiotics per unit using 2D TLC isolation, and single and co-activities were observed.
References
กรมพัฒนาแพทย์แผนไทยและการแพทย์ทางเลือก กระทรวงสาธารณสุข. 2554. เห็ดเป็นยาเพื่อสุขภาพ
ตามภูมิปัญญาของหมอพื้นบ้าน. สำนักการแพทย์พื้นบ้านไทย. องค์การสงเคราะห์ทหารผ่านศึก, กรุงเทพฯ
ชลดา จัดประกอบ, พรพรรณ เหล่าวชิระสุสรรณ และ เมธิน ผดุงกิจ. 2556. ฤทธิ์ต้านอนุมูลอิสระ ฤทธิ์ต้านการก่อ
กลายพันธุ์ของสารสกัดเห็ดหิ้งเกือกม้า. The 5th Annual Northeast Phamacy Research Conference of 2013, มหาวิทยาลัยมหาสารคาม, จังหวัดมหาสารคาม. (ประเทศไทย)
Bell, S. M., B. J. Gatus, J. N. Pham and D. L. Rafferty. 2006. Antibiotic susceptibility testing by the CDS
method. A manual for medical and veterinary laboratories. South Eastern Area Laboratory Services. 112 p.
Blagodatski, A., M. Yatsunskaya, V. Mikhailova, V. Tiasto, A. Kagansky and V.L. Katanaev. 2018.
Medicinal mushrooms as an attractive new source of natural compounds for future cancer therapy. Oncotarget 9(49): 29259-29274.
Jeon, T.I., C.H. Jung, J.Y. Cho, D.K. Park and J.H. Moon. 2013. Identification of an anticancer compound
against HT-29 cells from Phellinus linteus grown on germinated brown rice. Asian Pacific Journal of Tropical Biomedicine 3(10): 785-789.
Kim, S.H., J.M. Sung and T. C. Harrington. 1999. Identification of Phellinus linteus by morphological
characteristics and molecular analysis. Korean Journal of Mycology 27:337-340.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation
and cytotoxicity assays. J Immunol Methods. 65(2): 55-63.
Rizzo, D.M., P.T. Gieser and H.H. Burdsall. 2003. Phellinus coronadensis: a new species from southern
Arizona, USA. Mycologia 95(1): 74–79.
Shahverdi, A.R., F. Abdolpour, H.R. Monsef-Esfahani and H. Farsam. 2007. A TLC bioautographic assay for
the detection of nitrofurantoin resistance reversal compound. Journal of Chromatography B 850
(1-2) : 528–530.
Sonawane, H., S. Bhosle, and S. Garad. 2012. Antimicrobial activity of some species of Phellinus and
Ganoderma sample from western Ghats of India. International Journal of Pharmaceutical Sciences and Research 3(6): 1795-1799.
