Isolation and Identification of Actinomycetes from Soil for Controlling Certain Vegetable Insect Pests

Authors

  • Wichai Sorapongpaisal Center for Research and Academic Outreach, Faculty of Agriculture at Kampang Saen, Kasetsart University, Kamphaeng Saen Campus
  • Pabhop Sinchayakul Department of Entomology, Faculty of Agriculture at Kamphang Saen, Kasetsart University Kamphang Saen Campus
  • Araya Bunsak Faculty of Food and Agricultural Technology, Pibulsongkram Rajabhat University
  • Wipa Homhaul National Biological Control Research Center Lower Northern Regional Center, Naresuan University
  • Panisara Thepkusol National Biological Control Research Center Lower Northern Regional Center, Naresuan University

Keywords:

Actinomycetes, Metabolites, Spodoptera litura Plutella xylostella, Lipaphis erysimi

Abstract

Actinomycetes is bacteria producing various metabolites which some of them are highly efficient insecticides. Therefore, the research related to actinomycetes bacteria to control some vegetable insect pests was performed under the objective of isolation and identification of actinomycetes from soil for controlling certain insect pests of vegetables: Spodoptera litura, Plutella xylostella and Lipaphis erysimi. Soil samples were collected from 3, 3 and 9 sites in Ratchaburi, Kanchanaburi and Tak provinces, respectively, with 5 collecting spots of soil samples/site. Actinomyces were isolated using sequence dilution technique from soil suspension and spread on starch casein agar (SCA). The isolated actinomycetes colonies were picked up, cultured in glucose yeast malt (GYM) liquid media for 21 days and extracted by collecting supernatant of culture media for the efficacy test. The concentration of each crude extract was adjusted to 5% and applied on 10 larvae of S. litura at the 3rd instar. The high potential (mortality rate > 50%) of GYM crude extract of actinomycetes were screened and continuously used for determining their efficacy on P. xylostella and L. erysimi. The result reveled that the total of 477 actinomycetes isolates were found. The mortality rate above 50% on S. litura was detected on 9 GYM crude extracts of those actinomycetes isolates: KANAC011, RACAC007, RACAC032, RACAC05,5 TAKAC044, TAKAC094, TAKAC119, TAKAC178 and TAKAC181 and the highest efficacy (75.5% mortality at 72 hours after contact with the crude extract, HACE) was found from GYM crude extract of TAKAC094. Consequently, after applying the GYM crude extracts of those 9 isolates on P. xylostella and L. erysimi, the result showed that the highest potential to control P. xylostella (73.5 % mortality at 72 HACE) and L. erysimi (21.3% mortality at 72 HACE) was found from the GYM crude extracts of TAKAC181 and RACAC055, respectively

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Published

2022-07-12

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Research article Academic article and Review article