Incidence of Entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae and Associated Rice Pest Insects in Phitsanulok Province, Thailand
Keywords:
Beauveria bassiana, biological control, Metarhizium anisopliae, paddy field, surveyAbstract
This research investigated the incidence of Beauveria bassiana and Metarhizium anisopliae, entomopathogenic fungi (EPF), from infected rice and insect pests in paddy fields at Pitsanulok province, Thailand. The results of the survey of EPFs and insect pests (rice insect pests infected by those two EPFs) revealed that the abundance of some important Lepidoptera pests were 0.01–0.58 insects per site. Pyralid larvae, including the Yellow stem borer (Schoenobis bipunctifer) and Rice leaffolder (Cnapholochrocis medinalis) were mainly found. The dead insects that were infested with B. bassiana or M. anisopliae included the green leafhopper (Nephotettix virescen), and other members of Hemiptera, including plant-leafhoppers, as well as members of Coleoptera, Lepidoptera, and Diptera. Isolation and identification of B. bassiana revealed it had white colonies, filamentous and had definite cell walls. Under the same laboratory condition, M. anisopliae had hyaline to greenish hyphae that formed a sporodochium where conidiogenous cells were born. The basipetally produced chains adhered in distinct cylindrical columns with rounded ends, and were green (phialospores) in color
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