Potential of Combination of Trichoderma asperellum strain Cb-pin01 and Streptomyces sp. Strain KPS-E004 in Controlling M. enterolobii of Chili
Keywords:
Root-knot nematode in chili, biocontrol, Trichoderma sp., Streptomyces sp.Abstract
Meloidogyne enterolobii causes significant yield loss in chili (Capsicum frutescens L.). This research investigated the control potential of co-inoculation of chili with the T. asperellum strain Cb-pin01 and Streptomyces sp. strain KPS-E004 on the growth and symbiotic performance of M. enterolobii challenged chili. From the results of laboratory tests, the mixture of two strains reduced the egg hatch rate, increased the juvenile mortality rate and inhibited root penetration of infective second-stage juvenile of M. enterolobii up to 75.33%, 62.44% and 98.67%, respectively when compared to the control. The test with peppers in greenhouse conditions revealed that the mixture of two strains reduced the number of egg mass, reduced the number of second-stage juveniles in soil and suppressed root knot disease up to 71.05%, 75.68% and 50%, respectively when compared to positive control. The control efficiency of co-inoculation of both strains in suppressing root knot disease also increased up to 150.02% as compared to inoculation of single strain of Cb-pin01. Moreover, the mixtures promoted the growth of chili by increasing the shoot weight, shoot and root length and improving the yield of extra-large chili by 129.65%, 139.63%, 122.89% and 165.51%, respectively (compared to negative control). Based on the results of this study, T. asperellum strain Cb-pin01 and Streptomyces sp. strain KPS-E004 may apply together to control root-knot disease in chili plants.
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