DNA Markers Associated with Seed Dormancy in Zombi Pea [Vigna vexillata (L.) A. Rich]
Keywords:
Zombi pea, Vigna vexillata, seed dormancy, bulked segregant analysis, QTLAbstract
A decrease in or a loss of seed dormancy is a key trait in domestication of cereal and legume crops. It provides the uniform germination of seeds which is relevant for crop production. Although seed dormancy is disadvantage for crop cultivation, it can be exploited for improving resistance to pre-harvest spouting causing by excessive rain and humidity. Zombi pea (Vigna vexillata (L.) A. Rich) is an underutilized legume crop that adapts well to several climatic and environmental conditions. This research aimed to identify DNA marker(s) associated with seed dormancy in zombi pea. An F2 population of 94 plants developed from a cross between "TVNu 240" (cultivated) and "TVNu 1623" (wild) was used to identify DNA markers associated with seed dormancy. Broad-sense heritability estimated for seed dormancy in the population was 62.99 %. Bulk segregant analysis showed that simple sequence repeat (SSR) markers VvSSR-39, VvSSR-48 and VvSSR-71 were associated with seed dormancy. Single regression analysis explained 15.15 %, 13.06 % and 11.49%, of seed dormancy variation in the F2 population, respectively. QTL mapping identified a single QTL for the seed dormancy between the markers VvSSR-39 and VvSSR-71. The QTL explained 18.29 % of the trait variation. The results provide the basis for gene cloning of the seed dormancy in zombi pea
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