Validation of a Molecular Marker for Heat Tolerance Selection in Improved Aromatic Rice (Oryza sativa L.)
Keywords:
Rice, Heat tolerance, Breeding, Spikelet fertility, Molecular markerAbstract
The validation of a molecular marker for heat tolerance selection was conducted in 13 BC2F3 rice lines derived from a backcrossing between the aromatic recipient parent ‘RGD13215-MS13-MS19-10-4-MS26’ (RGD) and the well-known heat tolerance donor ‘Nagina-22’ (N22). The experiments were carried out under greenhouse condition at 30-52°C and natural field condition at 22-37°C. At the reproductive stage, three developing panicles/plant/replication were tagged when the length between the flag leaf and the second leaf was 5 cm. The same panicles were counted for seed set after harvesting. We used spikelet fertility (SF) as the heat-tolerant index in this study. The results showed that the SF of the seven introgression lines with N22 allele at the targeted locus were significantly higher than that of the six lines with RGD allele in both conditions. This suggested that the newly developed marker was effective and trustable for heat tolerance selection from N22 in this background. The marker will be validated in other populations with different backgrounds and in germplasm accessions for its potential in application in broader germplasm.
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